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Anti-PC1/3 antibody (ab3532)

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Overview

Product name

Anti-PC1/3 antibody
See all PC1/3 products (7) ...

Description

Rabbit polyclonal to PC1/3

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse

Predicted to work with

Rat, Cow

Immunogen

Synthetic Peptide: S(111)VQKDSALDLFNDPMWN(127). Synthetic peptide corresponds to amino acid residues 111-127 from mouse PC1. This sequence is 88% and 82% conserved bovine and rat, respectively.

SVQKDSALDL FNDPMWN

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

WB: mouse pituitary extract

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

PBS, 1 mg/ml BSA, 0.05% sodium azide

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Primary antibody notes

The subtilisin-like Prohormone Convertase (PC) family is a group of cellular enzymes that cleave most prohormones and neuropeptide precursors. Numerous other cellular proteins, some viral proteins, and bacterial toxins that are transported by the constitutive secretory pathway are also targeted for maturation by PCs. PC family members share structural similarities, which include a heterogeneous ~10 kDa amino-terminal proregion, a highly conserved ~55 kDa subtilisin-like catalytic domain, and carboxyl-terminal domain that is heterogeneous in length and sequence. These enzymes become catalytically active following proregion cleavage within the appropriate cellular compartment. The subcellular localization of PC family members varies. Immunolocalization studies show that PC1 is found in the perinuclear region as well as the trans-Golgi network, whereas PC2 can be found in the trans-Golgi network as well as diffusely distributed in the peripheral cytoplasm.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes
Neuroscience >> Neurology process >> Metabolism
Signal Transduction >> Growth Factors/Hormones >> Hormones

  • Western blot - PC1/3 antibody (ab3532)Western blot - PC1/3 antibody (ab3532) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab3532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 0.4 µg/mlDetects a band of approximately 87 kDa (predicted molecular weight: 80 kDa).

Target

Function

Involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. Substrates include POMC, renin, enkephalin, dynorphin, somatostatin and insulin.

Involvement in disease

Defects in PCSK1 are the cause of proprotein convertase 1 deficiency (PC1 deficiency) [MIM:600955]. PC1 deficiency is characterized by obesity, hypogonadism, hypoadrenalism, reactive hypoglycemia as well as marked small-intestinal absorptive dysfunction It is due to impaired processing of prohormones.

Sequence similarities

Belongs to the peptidase S8 family. Furin subfamily.

Cellular localization

Cytoplasmic vesicle > secretory vesicle. Localized in the secretion granules.

Target information above from: UniProt accessionP29120 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • BDP antibody
  • EC antibody
  • NEC 1 antibody
  • NEC1 antibody
  • NEC1_HUMAN antibody
  • Neuroendocrine convertase 1 antibody
  • PC1 antibody
  • PC3 antibody
  • PCSK1 antibody
  • Prohormone convertase 1 antibody
  • Prohormone convertase 1/3 antibody
  • Prohormone convertase 3 antibody
  • Proprotein convertase 1 antibody
  • SPC3 antibody
see all

Database links

see all

Anti-PC1/3 antibody images:

  Western blot - PC1/3 antibody (ab3532)

Western blot - PC1/3 antibody (ab3532)

Anti-PC1/3 antibody (ab3532) at 1 µg/ml + Spinal Cord (Mouse) Tissue Lysate at 10 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 80 kDa
Observed band size : 87 + 68 kDa (why is the actual band size different from the predicted?)
Additional bands at : 35 kDa,60 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 4 minutes

PC1/3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of PC1/3 due to the presence of a 27 amino acid signal peptide and an 83 amino acid propeptide.

References for Anti-PC1/3 antibody (ab3532)

This product has been referenced in:

  • Gagnon Jet al. Expression of PCSK1 (PC1/3), PCSK2 (PC2) and PCSK3 (furin) in mouse small intestine. Regul Pept 152:54-60 (2009).Read more (PubMed: 18706454) »

See 1 publication for this product

Publishing research using ab3532? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"