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PC12 (Rat adrenal pheochromocytoma cell line) Cytoplasmic Lysate (ab14883)

Q&A (2)

Overview

  • Product namePC12 (Rat adrenal pheochromocytoma cell line) Cytoplasmic LysateSee all PC12 lysates ...
  • General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • BackgroundGenerated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.

    • Applications

  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for PC12 (Rat adrenal pheochromocytoma cell line) Cytoplasmic Lysate (ab14883)

    ab14883 has not yet been referenced specifically in any publications.

    Publishing research using ab14883 ? Please let us know so that we can cite the reference in this datasheet

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    We would like to proceed and purchase the ab9794. When we will do the Western I will send you an image of the gel.  I will wait for your email with the discount code.  

    DISCOUNT CODE:xxxx Expiration date: dd.mm.2012 I am very pleased to hear you would like to use ab9794 and send us an image of your results. This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Ab...

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    We are looking for a positive control. Can you recommend one?

    Thank you very much for your call. Firstly, I would like to mention that we also have a PC12 cell lysate available that could be used as positive control for ab108383, as stated on the datasheet. I just point this out as a lysate may be of use as a con...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"