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PC12 (Rat adrenal pheochromocytoma cell line) Nuclear Lysate (ab14884)

Q&A (4)

Overview

  • Product namePC12 (Rat adrenal pheochromocytoma cell line) Nuclear LysateSee all PC12 lysates ...
  • General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.90
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT
    Note: With Protease inhibitors
  • Concentration information loading...
  • Research Areas
  • BackgroundGenerated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.

    • Applications

  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • References for PC12 (Rat adrenal pheochromocytoma cell line) Nuclear Lysate (ab14884)

    ab14884 has not yet been referenced specifically in any publications.

    Publishing research using ab14884 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab14884

    Displaying 1 - 4 of 4 results for Q&A

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    Thank you for your prompt reply. Please send the HeLa nuclear lysate that you described; just to confirm it is a nuclear lysate and not a cell lysate? I appreciate your help and patience with this matter.

    Thank you for your reply.

    The product ab29545 is a whole cell lysate not a nuclear lysate. The only nuclear lysate we have in stock is the ab14655that you currently have.

    If you still wish for me to send you ab29545, the whole cell lys...

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    I must have misunderstood you, I loaded 18uL per well (I had 10uL of HeLa nuclear lysate and added 10uL of 2x protein loading buffer to a total volume of 20ul of which I loaded 18ul per well). The wells themselves if loaded carefully can hold 22uL of samp...

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    Thank you for that information.

    I do not think adding 4ul more will increase your signal and since you can only load a maximum of 22ul/well, I would like to send you ab29545 as a free of charge replacement. This HeLa cell lysate is already in sa...

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    I loaded 2.5mg/ml of total protein (the original concentration) I also tried lower concentrations (0.5 mg/mL and 0.25 mg/mL) and saw signal only in the PC12 nuclear lysate of corresponding concentrations. I assume that if the PC12 lysate showed a clean si...

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    Thank you for your reply.

    I appreciate your patience in this matter, but when you say you loaded 2.5mg/ml of total protein, how many microlitres of lysate is that? I ask, as we send 100ug of total protein at at concentration of 2.5mg/ml (therefo...

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    On January 10, 2012 I placed an order for HeLa and PC12 nuclear lysates (order confirmation # **********). I ran both lysates at 2.5 mg/ml (original concentration) and stained the blot with three different antibodies; among them with a anti-TATA binding p...

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    Thank you for contacting Abcam.

    I am sorry about the problems you have been having with ab14655, HeLa cell lysate. I was wondering if you could tell me how much total protein you loaded of the cell lysate. I ask as there does seem to be a signal...

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