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PC12 (Rat adrenal pheochromocytoma cell line) Nuclear Lysate - forskolin stimulated (ab14885)

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Overview

  • Product namePC12 (Rat adrenal pheochromocytoma cell line) Nuclear Lysate - forskolin stimulatedSee all PC12 lysates ...
  • General notesTo ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC. The protein content has been determined by a Bradford-based assay. The PC12 nuclear extracts have been collected after a 30-minute incubation with or without forskolin (20 µM).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.50
    Constituents: 0.48% HEPES, 2.34% Sodium chloride, 20% Glycerol, 0.003% EDTA, 0.042% Sodium fluoride, 0.0002% Sodium molybdate, 0.018% Sodium orthovanadate, 0.216% Beta glycerophosphate, 0.015% DTT, 0.37% PBS
    Note: With Protease inhibitor
  • Concentration information loading...
  • Research Areas
  • BackgroundGenerated by Greene and Tischler (1976) from a transplantable rat adrenal pheochromocytoma line, PC12 cells respond reversibly to nerve growth factor (NGF) and therefore have a neuronal phenotype, making the line a useful model system for neuronal differentiation. They produce catecholamines (dopamine, norepinephrine), neurotransmitters, NPP and are therefore used in studies of differentiation, neurobiochemistry and neurobiology.

    • References for PC12 (Rat adrenal pheochromocytoma cell line) Nuclear Lysate - forskolin stimulated (ab14885)

    ab14885 has not yet been referenced specifically in any publications.

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    Is stimulation with forskolin equivalent to NGF stimulation?

    Thank you for your enquiry. Forskolin is a compound that activates the cAMP/PKA-dependent signaling pathway; it activates adenylate cyclase and hence elevates intracellular levels of the second messenger cAMP. cAMP controls metabolism, the cytoskeleto...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"