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PC4 protein (Mutated S17 + S13 + S15 + S19) (Human) (ab81957)

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Overview

  • Product namePC4 protein (Mutated S17 + S13 + S15 + S19) (Human)See all PC4 proteins and peptides ...
  • Protein descriptionRecombinant full length Human PC4 protein (serine mutations, 127 amino acids) isolated from an E. coli strain that carries the coding sequence of the protein under the control of T7 promoter, Predicted MWt 14 kDa (Swiss-Prot P53999).
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab81957 is purified by conventional chromatography.
  • Biological activity1 unit equals 1 nanogram of purified protein.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab81957 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    Functional Studies
    Gel supershift assays
  • Application notesGSA: Use 1 unit in a 20 µl reaction.
    SDS-PAGE: Use at an assay dependent dilution.

    ab81957 has been utilized for in vitro function studies, including transcription, DNA replication, in vitro phosphorylation, gel mobility shift assay, protein-protein interactions and as a substrate for in vitro acetylation. 20 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Activated RNA polymerase II transcriptional coactivator p15Interferon related developmental regulator 1MGC102747
        p14P15PC4PC4 LSBPositive cofactor 4RPO2TC1SUB1SUB1 homologTCP4_HUMAN
      see all
  • FunctionGeneral coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA).
  • Sequence similaritiesBelongs to the transcriptional coactivator PC4 family.
  • Post-translational
    modifications    Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P53999 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    References for PC4 protein (Mutated S17 + S13 + S15 + S19) (Human) (ab81957)

    ab81957 has not yet been referenced specifically in any publications.

    Publishing research using ab81957 ? Please let us know so that we can cite the reference in this datasheet

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