PC4 protein (Mutated S17 + S13 + S15 + S19) (Human) (ab81957)
Constituents: 20% Glycerol, 20mM Tris HCl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
Our Abpromise guarantee covers the use of ab81957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Gel supershift assays|
SDS-PAGE: Use at an assay dependent dilution.
ab81957 has been utilized for in vitro function studies, including transcription, DNA replication, in vitro phosphorylation, gel mobility shift assay, protein-protein interactions and as a substrate for in vitro acetylation. 20 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Activated RNA polymerase II transcriptional coactivator p15Interferon related developmental regulator 1MGC102747
- p14P15PC4PC4 LSBPositive cofactor 4RPO2TC1SUB1SUB1 homologTCP4_HUMAN
modificationsActivity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region.
References for PC4 protein (Mutated S17 + S13 + S15 + S19) (Human) (ab81957)
ab81957 has not yet been referenced specifically in any publications.