![Western blot - Anti-PCB antibody [10G7BF7] (ab123451) image](#PCB-Primary-antibodies-ab123451-1.gif)
![Immunocytochemistry - Anti-PCB antibody [10G7BF7] (ab123451) image](#PCB-Primary-antibodies-ab123451-3.gif)
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Product name
Anti-PCB antibody [10G7BF7]
See all PCB products (5) ...
Description
Mouse monoclonal [10G7BF7] to PCB
Tested applications
In-Cell ELISA, WB, ICCmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Purified PCB from Cow liver.
Positive control
HepG2, Rat H9C2, Rat H4IIE, Mouse MEF cell lysates; Purified Cow liver PCB; HDFn cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HBS
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purity is near homogeneity as judged by SDS-PAGE. ab123451 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
10G7BF7
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Metabolic signalling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Metabolic signalling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab123451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
In-Cell ELISA: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 130 kDa.
ICC: Use a concentration of 1 µg/ml
Target
Function
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Involvement in disease
Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP11498
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- MS774 antibody
- MS790 antibody
see all
Database links
- Entrez Gene: 5091 Human
- Entrez Gene: 18563 Mouse
- Entrez Gene: 25104 Rat
- Omim: 608786 Human
- SwissProt: P11498 Human
- SwissProt: Q05920 Mouse
- SwissProt: P52873 Rat
- Unigene: 89890 Human
see all
Anti-PCB antibody [10G7BF7] images:
Western blot - Anti-PCB antibody [10G7BF7] (ab123451)
![Western blot - Anti-PCB antibody [10G7BF7] (ab123451)](/ps/datasheet/images/123/ab123451/PCB-Primary-antibodies-ab123451-1.gif)
All lanes : Anti-PCB antibody [10G7BF7] (ab123451) at 1 µg/ml
Lane 1 : HepG2 cell lysate
Lane 2 : Hela
Lane 3 : H9C2
Lane 4 : MEF
Lane 5 : Recombinant PC
Lysates/proteins at 15 µg per lane.
Secondary
Goat-anti-Mouse HRP at 1/3000 dilution
Predicted band size : 130 kDa
Immunocytochemistry - Anti-PCB antibody [10G7BF7] (ab123451)
![Immunocytochemistry - Anti-PCB antibody [10G7BF7] (ab123451)](/ps/datasheet/images/123/ab123451/PCB-Primary-antibodies-ab123451-3.gif)
ab123451 at 1µg/ml staining PCB in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-PCB antibody [10G7BF7] (ab123451)
ab123451 has not yet been referenced specifically in any publications.
Publishing research using ab123451? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"