![Immunocytochemistry/ Immunofluorescence - PCB antibody [3H2AD9 ] (ab110314) image](#PCB-Primary-antibodies-ab110314-4.jpg)
![Immunoprecipitation - Anti-PCB antibody [3H2AD9 ] (ab110314) image](#PCB-Primary-antibodies-ab110314-2.gif)
![Flow Cytometry - PCB antibody [3H2AD9 ] (ab110314) image](#PCB-Primary-antibodies-ab110314-3.gif)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Lysates
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-PCB antibody [3H2AD9 ]
See all PCB products (7) ...
Description
Mouse monoclonal [3H2AD9 ] to PCB
Tested applications
ICC/IF, Flow Cyt, IP, In-Cell ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human
Immunogen
Purified pyruvate carboxylase from bovine liver
Positive control
Human fibroblast and HeLa cells; Human liver tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HBS
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
ab110314 as produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation.
Clonality
Monoclonal
Clone number
3H2AD9
Isotype
IgG1
Light chain type
kappa
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Energy transfer pathways >> Energy Metabolism
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Carbohydrate metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Metabolism >> Energy Metabolism
Applications
Show applications key
Our Abpromise guarantee covers the use of ab110314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 5 µg/ml. For 2 hours.
Flow Cyt: Use a concentration of 1 µg/ml.
IP: Use at an assay dependent dilution.
In-Cell ELISA: Use a concentration of 5 µg/ml.
Target
Function
Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.
Pathway
Carbohydrate biosynthesis; gluconeogenesis.
Involvement in disease
Defects in PC are the cause of pyruvate carboxylase deficiency (PC deficiency) [MIM:266150]. PC deficiency leads to lactic acidosis, mental retardation and death. It occurs in three forms: mild or type A, severe neonatal or type B, and a very mild lacticacidemia.
Sequence similarities
Contains 1 ATP-grasp domain.
Contains 1 biotin carboxylation domain.
Contains 1 biotinyl-binding domain.
Contains 1 carboxyltransferase domain.
Cellular localization
Mitochondrion matrix.
Target information above from: UniProt accessionP11498
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- mitochondrial antibody
- MS774 antibody
- MS790 antibody
see all
Database links
- Entrez Gene: 338471 Cow
- Entrez Gene: 5091 Human
- Entrez Gene: 18563 Mouse
- Entrez Gene: 25104 Rat
- Omim: 608786 Human
- SwissProt: Q29RK2 Cow
- SwissProt: P11498 Human
- SwissProt: Q05920 Mouse
see all
Anti-PCB antibody [3H2AD9 ] images:
Immunocytochemistry/ Immunofluorescence - PCB antibody [3H2AD9 ] (ab110314)
![Immunocytochemistry/ Immunofluorescence - PCB antibody [3H2AD9 ] (ab110314)](/ps/datasheet/images/110/ab110314/PCB-Primary-antibodies-ab110314-4.jpg)
Upper image shows: Immunocytochemistry analysis using ab110314 at 5µg/ml staining PCB in Human fibroblast cells (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: The target protein locates to the mitochondrial matrix.
Lower image shows cells co-stained with an antibody against PDH (green), an enzyme also located in the mitochondrial matrix. The composite image shows an identical mitochondrial pattern for both antibodies indicated by merged orange color.
Immunoprecipitation - Anti-PCB antibody [3H2AD9 ] (ab110314)
![Immunoprecipitation - Anti-PCB antibody [3H2AD9 ] (ab110314)](/ps/datasheet/images/110/ab110314/PCB-Primary-antibodies-ab110314-2.gif)
Detection of ab110314 by immunoprecipitation staining of 130kDa PCB in human liver lysate.
Flow Cytometry - PCB antibody [3H2AD9 ] (ab110314)
![Flow Cytometry - PCB antibody [3H2AD9 ] (ab110314)](/ps/datasheet/images/110/ab110314/PCB-Primary-antibodies-ab110314-3.gif)
Flow cytometric analysis using ab110314 at 1µg/ml staining PCB in HeLa cells (blue). Isotype control antibody (red).
References for Anti-PCB antibody [3H2AD9 ] (ab110314)
ab110314 has not yet been referenced specifically in any publications.
Publishing research using ab110314? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"