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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab74793? Please let us know so that we can cite the reference in this datasheet
ab74793 has been referenced in 2 publications.
- Sutherland JM et al. Suppressor of cytokine signaling 4 (SOCS4): moderator of ovarian primordial follicle activation. J Cell Physiol 227:1188-98 (2012). WB, IHC-P; Mouse. PubMed: 21604262
- Li Z et al. Proteomic profile of primary isolated rat mesangial cells in high-glucose culture condition and decreased expression of PSMA6 in renal cortex of diabetic rats. Biochem Cell Biol 88:635-48 (2010). WB; Rat. PubMed: 20651835
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - PCBP1 antibody (ab74793)
Anti-PCBP1 antibody (ab74793) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 37 kDa
Observed band size : 37 kDa
Immunocytochemistry/ Immunofluorescence-PCBP1 antibody(ab74793)
ICC/IF image of ab74793 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74793, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP1 antibody (ab74793)
ab74793 at 5µg/ml staining PCBP1 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue).
Western blot - Anti-PCBP1 antibody (ab74793)
All lanes : Anti-PCBP1 antibody (ab74793) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from MEF cells
Lane 2 : Whole cell lysate prepared from F9 cells
Lane 3 : Whole cell lysate prepared from GC1 cells
Lane 4 : Whole cell lysate prepared from NIH-3T3 cells
Lysates/proteins at 30 µg per lane.
Secondary
HRP conjugated pig anti-rabbit polyclonal
developed using the ECL technique
Predicted band size : 37 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
Image courtesy of an anonymous Abreview.
Anti-PCBP1 antibody for Western blot in Human (74793)
Anti-PCBP1 antibody for Western blot in Mouse (74793)
PCBP1 antibody for WB in Human (74793)
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