Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Overview
Product name
Anti-PCBP1 antibody
See all PCBP1 products (4) ...
Description
Rabbit polyclonal to PCBP1
Tested applications
IHC-P, ICC/IF, WB, ELISAmore details
Cross reactivity
Reacts with
Rat, Human
Predicted to work with
Mouse, Cow, Dog
Immunogen
A synthetic peptide corresponding to a region of Human PCBP1
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution.
ICC/IF: Use a concentration of 1 mg/ml
WB: Use a concentration of 1.0 µg/mlDetects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).
ELISA: Use at an assay dependent dilution.
Target
Function
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC.
Tissue specificity
Abundantly expressed in skeletal muscle, thymus and peripheral blood leukocytes while a lower expression is observed in prostate, spleen, testis, ovary, small intestine, heart, liver, adrenal and thyroid glands.
Sequence similarities
Contains 3 KH domains.
Post-translational
modifications
Phosphorylated; lowers poly(rC)-binding activity.
Cellular localization
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
Target information above from: UniProt accessionQ15365
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha-CP1 antibody
- Heterogeneous nuclear ribonucleoprotein E1 antibody
- heterogenous nuclear ribonucleoprotein E1 antibody
see all
Database links
- Entrez Gene: 481417 Dog
- Entrez Gene: 5093 Human
- Entrez Gene: 23983 Mouse
- Entrez Gene: 500242 Rat
- Omim: 601209 Human
- SwissProt: Q15365 Human
- SwissProt: P60335 Mouse
- Unigene: 2853 Human
see all
Anti-PCBP1 antibody images:
Western blot - PCBP1 antibody (ab74793)
Anti-PCBP1 antibody (ab74793) at 1 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 37 kDa
Observed band size : 37 kDa
Immunocytochemistry/ Immunofluorescence-PCBP1 antibody(ab74793)
ICC/IF image of ab74793 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74793, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCBP1 antibody (ab74793)
ab74793 at 5µg/ml staining PCBP1 in Human spleen tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue).
References for Anti-PCBP1 antibody (ab74793)
This product has been referenced in:
- Li Zet al. Proteomic profile of primary isolated rat mesangial cells in high-glucose culture condition and decreased expression of PSMA6 in renal cortex of diabetic rats. Biochem Cell Biol 88:635-48 (2010). WB; Rat.Read more (PubMed: 20651835) »
See 1 publication for this product
Publishing research using ab74793? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"