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Products:Microbiology >> Interspecies Interaction >> Host Virus Interaction
Overview
Product name
Anti-PCBP2/hnRNP E2 antibody
See all PCBP2/hnRNP E2 products (4) ...
Description
Mouse monoclonal to PCBP2/hnRNP E2
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 1-363 of Human PCBP2/hnRNP E2 (AAH01155) with a 26kDa tag
Positive control
K562 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Epigenetics and Nuclear Signaling >> DNA / RNA >> Translation >> Ribosome
Microbiology >> Interspecies Interaction >> Host Virus Interaction
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ELISA: Use at an assay dependent dilution.
sELISA: Use at an assay dependent dilution. Detection limit is approximately 3ng/ml as a capture antibody when used against the immunogen.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. Major cellular poly(rC)-binding protein. Binds also poly(rU). Negatively regulates cellular antiviral responses mediated by MAVS signaling. It acts as an adapter between MAVS and the E3 ubiquitin ligase ITCH, therefore triggering MAVS ubiquitinationa and degradation.
Tissue specificity
Detected in all tissues examined.
Sequence similarities
Contains 3 KH domains.
Post-translational
modifications
Phosphorylated. The non-phosphorylated form(s) exhibited the strongest poly(rC)-binding activity.
Cellular localization
Nucleus. Cytoplasm. Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm.
Target information above from: UniProt accessionQ15366
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha CP2 antibody
- Alpha-CP2 antibody
- alphaCP-2 antibody
see all
Database links
- Entrez Gene: 5094 Human
- Omim: 601210 Human
- SwissProt: Q15366 Human
- Unigene: 546271 Human
Anti-PCBP2/hnRNP E2 antibody images:
Western blot - PCBP2/hnRNP E2 antibody (ab77323)
Anti-PCBP2/hnRNP E2 antibody (ab77323) at 5 µg/ml + K562 cell lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
developed using the ECL technique
Predicted band size : 39 kDa
Observed band size : 39 kDa
Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.
Sandwich ELISA - PCBP2/hnRNP E2 antibody (ab77323)
Sandwich ELISA using ab77323 at 3ng/ml as a capture antibody against the recombinant immunogen at the indicated concetrations.
References for Anti-PCBP2/hnRNP E2 antibody (ab77323)
ab77323 has not yet been referenced specifically in any publications.
Publishing research using ab77323? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"