Anti-PCCB antibody (ab186836)
Key features and details
- Rabbit polyclonal to PCCB
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-PCCB antibody
See all PCCB primary antibodies -
Description
Rabbit polyclonal to PCCB -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein corresponding to Human PCCB aa 1-539.
Database link: P05166 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB |
1/500 - 1/2000. Predicted molecular weight: 58 kDa.
|
Notes |
---|
ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 58 kDa. |
Target
-
Pathway
Metabolic intermediate metabolism; propanoyl-CoA degradation; succinyl-CoA from propanoyl-CoA: step 1/3. -
Involvement in disease
Defects in PCCB are the cause of propionic acidemia type II (PA-2) [MIM:606054]. PA-2 is a life-threatening disease characterized by episodic vomiting, lethargy and ketosis, neutropenia, periodic thrombocytopenia, hypogammaglobulinemia, developmental retardation, and intolerance to protein. -
Sequence similarities
Belongs to the AccD/PCCB family.
Contains 1 carboxyltransferase domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
-
Database links
- Entrez Gene: 5096 Human
- Entrez Gene: 66904 Mouse
- Omim: 232050 Human
- SwissProt: P05166 Human
- SwissProt: Q99MN9 Mouse
- Unigene: 63788 Human
- Unigene: 335385 Mouse
-
Alternative names
- DKFZp451E113 antibody
- PCCase subunit beta antibody
- pccB antibody
see all
Images
-
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186836. Blue DAPI for nuclear staining.
-
All lanes : Anti-PCCB antibody (ab186836) at 1/500 dilution
Lane 1 : Extracts from HT29 cell line
Lane 2 : Extracts from mouse liver
Lane 3 : Extracts from mouse kidney
Lane 4 : Extracts from mouse heart
Predicted band size: 58 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab186836 has not yet been referenced specifically in any publications.