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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Overview
Product name
Anti-PCK2 antibody
See all PCK2 products (4) ...
Description
Goat polyclonal to PCK2
Specificity
This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1.
Tested applications
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Cow, Dog, Pig
Immunogen
Synthetic peptide: KPWKPGDKEPCAH, corresponding to Internal sequence amino acids 407-419 of Human PCK2
KPWKPGDKEP CAH
Positive control
Human liver lysate and HepG2
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of carbohydrates
Signal Transduction >> Metabolism >> Mitochondrial
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> PKC
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
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Western blot - PCK2 antibody (ab40843)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab40843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.1 - 0.3 µg/ml.Predicted molecular weight: 71 kDa.
Target
Relevance
PCK2 is a a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described.
Cellular localization
Mitochondrial
Alternative names
- EC 4.1.1.32 antibody
- GTP mitochondrial precursor antibody
- HGNC:8725 antibody
see all
Database links
- Entrez Gene: 5106 Human
- Entrez Gene: 74551 Mouse
- Entrez Gene: 361042 Rat
- Omim: 261650 Human
- SwissProt: Q16822 Human
- SwissProt: Q8BH04 Mouse
Anti-PCK2 antibody images:
Western blot - PCK2 antibody (ab40843)
Anti-PCK2 antibody (ab40843) at 0.1 µg/ml + Human HepG2 lysate (35µg protein in RIPA buffer).
Predicted band size : 71 kDa
Observed band size : 71 kDa
Primary incubation was 1 hour. Detected by chemiluminescence.
References for Anti-PCK2 antibody (ab40843)
This product has been referenced in:
- Wendel AAet al. Glycerol-3-phosphate acyltransferase 1 deficiency in ob/ob mice diminishes hepatic steatosis but does not protect against insulin resistance or obesity. Diabetes 59:1321-9 (2010). WB; Mouse.Read more (PubMed: 20200319) »
- Samuel VTet al. Fasting hyperglycemia is not associated with increased expression of PEPCK or G6Pc in patients with Type 2 Diabetes. Proc Natl Acad Sci U S A 106:12121-6 (2009). WB; Rat.Read more (PubMed: 19587243) »
See all 3 publications for this product
Publishing research using ab40843? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"