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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab72443? Please let us know so that we can cite the reference in this datasheet
ab72443 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - PCM1 antibody (ab72443)
All lanes : Anti-PCM1 antibody (ab72443) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Observed band size : 270 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with exposure times of 30 seconds
Immunoprecipitation - PCM1 antibody (ab72443)
Immunoprecipitation of PCM1 from 1mg HeLa whole cell lysate by 3 ug ab72443 followed by WB (20% of IP loaded):
Lane 1: PCM1 precipitate
Lane 2: Control IgG
For blotting immunoprecipitated PCM1, ab72443 was used at 1 ug/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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