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Products:Epigenetics and Nuclear Signaling >> Chromosome Structure >> Centromeres
Overview
Product name
Anti-PCM1 antibody
Description
Rabbit polyclonal to PCM1
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
A synthetic peptide corresponding to a region between residues 1925 and 1975 of human PCM1.
Positive control
HeLa whole cell lysate; 293T and mouse NIH3T3 cell lysates
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Epigenetics and Nuclear Signaling >> Chromosome Structure >> Centromeres
Applications
Show applications key
Our Abpromise guarantee covers the use of ab72443 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 270 kDa.
IP: Use a concentration of 2 - 5 µg/ml.
Target
Function
Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia.
Tissue specificity
Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.
Involvement in disease
Defects in PCM1 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving PCM1 is found in thyroid papillary carcinomas. Translocation t(8;10)(p21.3;q11.2) with RET links the protein kinase domain of RET to the major portion of PCM1.
Note=A chromosomal aberration involving PCM1 is found in a variety of hematological malignancies including atypical chronic myeloid leukemia (atypical CML) and T-cell lymphoma. Translocation t(8;9)(p22;p24) with JAK2 links the protein kinase domain of JAK2 to the major portion of PCM1.
Sequence similarities
Belongs to the PCM1 family.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cytoplasmic granule. Localizes to cytoplasmic granules which are enriched around the centrosome. This centrosomal enrichment requires microtubules and dynein. The majority of the protein dissociates from the centrosome during metaphase and subsequently localizes to the cleavage site in telophase.
Target information above from: UniProt accessionQ15154
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hPCM-1 antibody
- PCM-1 antibody
- pcm1 antibody
see all
Database links
- Entrez Gene: 5108 Human
- Entrez Gene: 18536 Mouse
- Omim: 600299 Human
- SwissProt: Q15154 Human
- SwissProt: Q9R0L6 Mouse
- Unigene: 491148 Human
- Unigene: 117896 Mouse
Anti-PCM1 antibody images:
Western blot - PCM1 antibody (ab72443)
All lanes : Anti-PCM1 antibody (ab72443) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Observed band size : 270 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with exposure times of 30 seconds
Immunoprecipitation - PCM1 antibody (ab72443)
Immunoprecipitation of PCM1 from 1mg HeLa whole cell lysate by 3 ug ab72443 followed by WB (20% of IP loaded):
Lane 1: PCM1 precipitate
Lane 2: Control IgG
For blotting immunoprecipitated PCM1, ab72443 was used at 1 ug/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
References for Anti-PCM1 antibody (ab72443)
ab72443 has not yet been referenced specifically in any publications.
Publishing research using ab72443? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"