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Western blot - PCM1 antibody (ab72443)
(enlarge)
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Immunoprecipitation - PCM1 antibody (ab72443)
(enlarge)
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Product Name
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PCM1 antibody
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PCM1
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Immunogen
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A synthetic peptide corresponding to a region between residues 1925 and 1975 of human PCM1.
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at a concentration of 2 - 5 µg/ml.
WB: 1/2000 - 1/10000. Detects a band of approximately 270 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa whole cell lysate; 293T and mouse NIH3T3 cell lysates
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Cellular localization
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Cytoplasmic
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Research areas
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Cell Biology >> Cell Cycle >> Cell Division >> Centromere
Chromatin and Nuclear Signaling >> Chromosome Structure >> Centromeres
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Relevance
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PCM1 is required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. It is expressed in blood, bone marrow, breast, lymph node, ovary and thyroid
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PCM1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PCM1 antibody images, references, products related to ab72443 and other tools.
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PCM1 antibody images:
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 Western blot - PCM1 antibody (ab72443)
All lanes : PCM1 antibody (ab72443) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Lane 5 : Mouse NIH3T3 cell lysate at 50 µg
Observed band size : 270 kDa (why is the actual band size different from the predicted?)
Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
Detection: Chemiluminescence with exposure times of 30 seconds
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Immunoprecipitation - PCM1 antibody (ab72443)
Immunoprecipitation of PCM1 from 1mg HeLa whole cell lysate by 3 ug ab72443 followed by WB (20% of IP loaded):
Lane 1: PCM1 precipitate
Lane 2: Control IgG
For blotting immunoprecipitated PCM1, ab72443 was used at 1 ug/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
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Search PubMed (MEDLINE) for references to PCM1
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PCM1 antibody - more information
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Customer reviews (feedback) regarding PCM1 antibody |
Customer FAQs regarding PCM1 antibody |
Protocols for PCM1 antibody |
Price and availability of products related to PCM1 antibody |
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PCM1 antibody - related products:
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PCM1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PCM1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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