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ab29 has been referenced in 41 publications.
Publishing research using ab29? Please let us know so that we can cite the reference in this datasheet
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Mouse monoclonal [PC10] to PCNA - Proliferation Marker (ab29) used in immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections;1/6000 for 2h at RT) on intestine of adult Zebra fish). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Incubation time: Secondary Antibody: Biotin conjugated goat anti mouse Igs (1/200). NB: The crypt nuclei on this image of zebrafish intestine, are positive for the PCNA/PC10 clone conforming to accepted localisation data for PCNA in other species.
Carl Hobbs, CARD, KCL, UK
Mouse monoclonal [PC10] to PCNA - Proliferation Marker (ab29) used in immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections; 1/6000 for 2h at RT) on Human Tissue sections (Paget's disease of the nipple). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Incubation time: Secondary Antibody: Biotin conjugated goat anti mouse Igs (1/200).
Carl Hobbs, CARD, KCL, UK
All lanes : Anti-PCNA antibody [PC10] - Proliferation Marker (ab29) at 5 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 29 kDa
Observed band size : 29 kDa
Exposure time : 4 minutes
Overlay histogram showing HeLa cells stained with ab29 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (
All lanes : Anti-PCNA antibody [PC10] - Proliferation Marker (ab29) at 1/5000 dilution
Lane 1 : Human 293 total cell extract
Lane 2 : Chicken DT40 total cell extract
Performed under reducing conditions.
Predicted band size : 29 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 seconds
Blocking for 2 hours in 5% milk. Primary antibody incubated for 1 hour.
Taken from an Abreview submitted by Javier di Noia
ab29 at a 1/500 dilution used in immunoprecipitation.
Lane 1: Beads only + HeLa whole cell lysate
Lane 2: Beads only + SupT1 whole cell lysate
Lane 3: Beads + ab29 + HeLa whole cell lysate
Lane 4: Beads + ab29 + SupT1 whole cell lysate
A 50KDa band is precipitated which is IgG heavy chain whilst the 30kDa band is PCNA.
This image is courtesy of an anonymous Abreview
ab29 at 1/6000 staining mouse embryo (day 17) liver and gut tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in Tris buffer was performed. The tissue was blocked before incubation with the antibody for 2 hours. A biotinylated goat polyclonal antibody was used as the secondary.
This image is courtesy of an Abreview submitted by Mr Carl hobbs
Mouse monoclonal [PC10] to PCNA - Proliferation Marker (ab29) used in immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections; 1/6000 for 2h at RT) on E6 developing chick brain). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Incubation time: Secondary Antibody: Biotin conjugated goat anti mouse Igs (1/200). NB: This image shows developing brain/overlying skin.
Carl Hobbs, CARD, KCL, UK
Immunohistochemistical staining (Formaldehyde/PFA-fixed paraffin-embedded sections) for PCNA antibody [PC10] - Proliferation Marker (ab29) on Rat Tissue sections (adult spinal cord DRG). Antigen retrieval step: Heat mediated. Blocking step: 1% BSA for 10 mins at RT. Primary Antibody used at 1/6000 for 2 minutes at RT. Secondary Antibody: Biotin labelled goat anti mouse Igs (1/200).
Carl Hobbs, CARD, KCL, London, UK
ICC/IF image of ab29 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab29, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HEK 293, HepG2 and MCF7 cells.
ab29 staining PCNA in mouse embryonic brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and permeabilized with 0.1% PBS-Tween before blocking with 5% BSA for 1 hour at 220C. The sample was incubated with primary antibody (1/500) in 5% BSA in 0.3% PBS-Triton-X100 for 14 hours at 220C. An Alexa Fluor®488-conjugated Goat polyclonal to mouse IgG was used as secondary antibody at 1/500 dilution.
This image is a courtesy of Anonymous Abreview
ICC/IF image of ab29 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab29, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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