Anti-PCNA antibody [PC10 (a.k.a. 3F81)] (Phycoerythrin) (ab93576)

Overview

• Product nameAnti-PCNA antibody [PC10 (a.k.a. 3F81)] (Phycoerythrin)See all PCNA primary antibodies ...
• Description
  Mouse monoclonal [PC10 (a.k.a. 3F81)] to PCNA (Phycoerythrin)
• ConjugationPhycoerythrin
• Tested applicationsIHC-Fr, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Protein A-Rat PCNA fusion obtained from pC2T.

• Positive controlAlcohol fixed MOLT-4 cells

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.2% BSA, PBS, 150mM Sodium chloride, pH 7.2
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone numberPC10 (a.k.a. 3F81)
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Cancer
  • Cell cycle
  • Cell division

  • Cancer
  • Tumor biomarkers
  • Tumor antigens

  • Tags & Cell Markers
  • Cell Type Markers
  • Replication

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • DNA Synthesis
  • Other

  • Epigenetics and Nuclear Signaling
  • DNA methylation
  • Methylated DNA Binding

  • Epigenetics and Nuclear Signaling
  • Chromatin Binding Proteins
  • DNA / RNA binding

  • Cell Biology
  • Cell Cycle
  • Markers

Applications

Target

• FunctionThis protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.
• Sequence similaritiesBelongs to the PCNA family.
• Post-translational
  modificationsUpon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
  Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.
• Cellular localizationNucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents.

Target information above from: UniProt accession P12004 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5111 Human
  • Entrez Gene: 18538 Mouse
  • Entrez Gene: 25737 Rat
  • Omim: 176740 Human
  • SwissProt: P12004 Human
  • SwissProt: P17918 Mouse
  • SwissProt: P04961 Rat
  • Unigene: 147433 Human

  • Unigene: 728886 Human
  • Unigene: 7141 Mouse
  • Unigene: 223 Rat

  see all

• Alternative names
  Cyclin antibodyDNA polymerase delta auxiliary protein antibodyHGCN8729 antibody

  MGC8367 antibodyMutagen-sensitive 209 protein antibodyOTTHUMP00000030189 antibodyOTTHUMP00000030190 antibodyPCNA antibodyPcna/cyclin antibodyPCNA_HUMAN antibodyPCNAR antibodyPolymerase delta accessory protein antibodyProliferating Cell Nuclear Antigen antibody

  see all

Anti-PCNA antibody [PC10 (a.k.a. 3F81)] (Phycoerythrin) images

• 

  Flow Cytometry - Anti-PCNA antibody [PC10 (a.k.a. 3F81)] (Phycoerythrin) (ab93576)
  Intracellular staining of Molt-4 cells with FITC conjugated anti-Human PCNA (PC10) (left), and ab93576 (right). Appropriate isotype controls were used (open histogram). Total cells were used for analysis.