Overview
- Product nameAnti-PCP4 antibody
- DescriptionMouse monoclonal to PCP4
- Tested applicationsWB, ELISA more details
- Species reactivityReacts with: Recombinant Fragment
Predicted to work with: Mouse, Rat, Cow, Human - Immunogen
Recombinant fragment with a tag: MSERQGAGAT NGKDKTSGEN DGQKKVQEEF DIDMDAPETE RAAVAIQSQF RKFQKKKAGS QS, corresponding to amino acids 1-62 of human PCP4 (NP_006189). The tag sequence is not included here.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS, pH 7.2 -
Concentration information loading... - PurityProtein A purified
- Clonality Monoclonal
- IsotypeIgG2a
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab76840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 7 kDa. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. |
| ELISA | ELISA: Use at an assay dependent dilution. |
Target
- Sequence similaritiesBelongs to the PCP4 family.
Contains 1 IQ domain. -
Database links
- Entrez Gene: 5121 Human
- Entrez Gene: 18546 Mouse
- Entrez Gene: 25510 Rat
- Omim: 601629 Human
- SwissProt: P48539 Human
- SwissProt: P63054 Mouse
- SwissProt: P63055 Rat
- Unigene: 80296 Human
- Unigene: 5023 Mouse
- Unigene: 9736 Rat
see all
Target information above from: UniProt accession
P48539
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Brain specific antigen PCP 4 antibodyBrain specific polypeptide PEP 19 antibodyBrain specific polypeptide PEP19 antibody
- Brain-specific antigen PCP-4 antibodyBrain-specific polypeptide PEP-19 antibodyOTTHUMP00000109191 antibodyPcp4 antibodyPCP4_HUMAN antibodyPEP19 antibodyPurkinje cell protein 4 antibody
see all
Anti-PCP4 antibody images
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Western blot - PCP4 antibody (ab76840)Anti-PCP4 antibody (ab76840) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 7 kDa
Observed band size : 33 kDa (why is the actual band size different from the predicted?) -
ELISA - PCP4 antibody (ab76840)Detection limit for the recombinant tagged PCP4 is approximately 1 ng/ml as a capture antibody.
References for Anti-PCP4 antibody (ab76840)
ab76840 has not yet been referenced specifically in any publications.
