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ab28770 has been referenced in 3 publications.
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All lanes : Anti-PCSK9 antibody (ab28770) at 0.2 µg/ml
Lane 1 : untransfected McA cell lysate.
Lane 2 : McA cell lysate transfected with wild type human PCSK9
Lane 3 : McA cell lysate transfected with S127R human PCSK9
Predicted band size : 74 kDa
Observed band size : 60,73 kDa (why is the actual band size different from the predicted?)
ICC/IF image of ab28770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28770, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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