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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-PCSK9 antibody
See all PCSK9 products (8) ...
Description
Goat polyclonal to PCSK9
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: CRSRHLAQASQELQ, corresponding to C terminal amino acids 679-692 of Human PCSK9
CRSRHLAQAS QELQ
Positive control
Human liver, pancreas and adipose lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab28770 was purified from goat serum by ammonium sulphate precipitation prior to immunogen affinity chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Signal Transduction >> Metabolism >> Lipid metabolism
Stem Cells >> Neural Stem Cells >> Intracellular
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Signal Transduction >> Metabolism >> Amino Acids
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.2 - 0.3 µg/ml.Predicted molecular weight: 74 kDa.
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Tissue specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain.
Post-translational
modifications
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ8NBP7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FH3 antibody
- HCHOLA3 antibody
- Hypercholesterolemia autosomal dominant 3 antibody
see all
Database links
- Entrez Gene: 255738 Human
- Omim: 607786 Human
- SwissProt: Q8NBP7 Human
- Unigene: 18844 Human
Anti-PCSK9 antibody images:
Western blot - PCSK9 antibody (ab28770)
All lanes : Anti-PCSK9 antibody (ab28770) at 0.2 µg/ml
Lane 1 : untransfected McA cell lysate.
Lane 2 : McA cell lysate transfected with wild type human PCSK9
Lane 3 : McA cell lysate transfected with S127R human PCSK9
Predicted band size : 74 kDa
Observed band size : 60,73 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence-PCSK9 antibody(ab28770)
ICC/IF image of ab28770 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28770, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-PCSK9 antibody (ab28770)
This product has been referenced in:
- Liu Met al. PCSK9 is not involved in the degradation of LDL receptors and BACE1 in the adult mouse brain. J Lipid Res 51:2611-8 (2010).Read more (PubMed: 20453200) »
- Lambert Get al. Plasma PCSK9 Concentrations Correlate with LDL and Total Cholesterol in Diabetic Patients and Are Decreased by Fenofibrate Treatment. Clin Chem : (2008). WB; Human.Read more (PubMed: 18436719) »
See all 3 publications for this product
Publishing research using ab28770? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"