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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-PCSK9 antibody
See all PCSK9 products (8) ...
Description
Rabbit polyclonal to PCSK9
Tested applications
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Mouse PCSK9.
(Peptide available as ab32727.)
Positive control
This antibody gave a positive signal in the following tissue lysates: Liver (Mouse) Kidney (Mouse) Liver (Rat) Kidney (Rat)
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Signal Transduction >> Metabolism >> Lipid metabolism
Stem Cells >> Neural Stem Cells >> Intracellular
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Signal Transduction >> Metabolism >> Amino Acids
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Western blot - PCSK9 antibody (ab31762)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 62 kDa (predicted molecular weight: 74 kDa).
Target
Function
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Tissue specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain.
Post-translational
modifications
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ8NBP7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FH3 antibody
- HCHOLA3 antibody
- Hypercholesterolemia autosomal dominant 3 antibody
see all
Database links
- Entrez Gene: 100102 Mouse
- Entrez Gene: 298296 Rat
- SwissProt: Q3UEH7 Mouse
- SwissProt: Q80W65 Mouse
- SwissProt: P59996 Rat
- SwissProt: Q5I6U6 Rat
- Unigene: 133268 Mouse
- Unigene: 19195 Rat
Anti-PCSK9 antibody images:
Western blot - PCSK9 antibody (ab31762)
All lanes : Anti-PCSK9 antibody (ab31762) at 1 µg/ml
Lane 1 : Liver (Mouse) Tissue Lysate
Lane 2 : Kidney (Mouse) Tissue Lysate
Lane 3 : Liver (Rat) Tissue Lysate
Lane 4 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 74 kDa
Observed band size : 62 kDa (why is the actual band size different from the predicted?)
ab31762 detects a band of ~62 kDa in liver and kidney lysates from mouse and rat. This corresponds to the cleaved form of PCSK9. ab31762 has not detected the 74 kDa proprotein form in the lysates tested.
References for Anti-PCSK9 antibody (ab31762)
ab31762 has not yet been referenced specifically in any publications.
Publishing research using ab31762? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"