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Products:Signal Transduction >> Metabolism >> Amino Acids
Overview
Product name
Anti-PCSK9 antibody
See all PCSK9 products (8) ...
Description
Rabbit polyclonal to PCSK9
Specificity
Detects both the zymogen and active forms of PC9. Smaller cleavage products are also detected.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic peptide based on the catalytic domain of human PC9.
Epitope
The epitope is C-terminal from the propeptide cleavage site.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Signal Transduction >> Metabolism >> Lipid metabolism
Stem Cells >> Neural Stem Cells >> Intracellular
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Signal Transduction >> Metabolism >> Amino Acids
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Immunocytochemistry/ Immunofluorescence - PCSK9 antibody (ab52754)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab52754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: 1/1000Predicted molecular weight: 74 kDa.(1/1000 when using colorimetric substrates and 1/5000 for chemiluminescent substrates. (pre-pro-peptide runs at 78-80 kDa, the mature form at 58 kDa))
Target
Function
May be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
Tissue specificity
Expressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
Involvement in disease
Defects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
Sequence similarities
Belongs to the peptidase S8 family.
Contains 1 peptidase S8 domain.
Post-translational
modifications
The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
Cellular localization
Secreted.
Target information above from: UniProt accessionQ8NBP7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FH3 antibody
- HCHOLA3 antibody
- Hypercholesterolemia autosomal dominant 3 antibody
see all
Database links
- Entrez Gene: 255738 Human
- Entrez Gene: 298296 Rat
- Omim: 607786 Human
- SwissProt: Q8NBP7 Human
- SwissProt: P59996 Rat
- SwissProt: Q5I6U6 Rat
- Unigene: 18844 Human
- Unigene: 19195 Rat
Anti-PCSK9 antibody images:
Immunocytochemistry/ Immunofluorescence - PCSK9 antibody (ab52754)
ICC/IF image of ab52754 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52754, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-PCSK9 antibody (ab52754)
ab52754 has not yet been referenced specifically in any publications.
Publishing research using ab52754? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"