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Immunocytochemistry/ Immunofluorescence - PCSK9 antibody (ab52754)
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Product Name
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PCSK9 antibody
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See all PCSK9 antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PCSK9
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Immunogen
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Synthetic peptide based on the catalytic domain of human PC9.
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Reacts with
(species key)
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Hu, Rat
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Specificity
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Detects both the zymogen and active forms of PC9. Smaller cleavage products are also detected.
We have a range of domain specific antibodies for this target. For a full list please see all PCSK9 antibodies
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Epitope
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The epitope is C-terminal from the propeptide cleavage site.
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric substrates and 1/5000 for chemiluminescent substrates. Predicted molecular weight: 74 kDa (pre-pro-peptide runs at 78-80 kDa, the mature form at 58 kDa)
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Secreted
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Research areas
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Cell Biology >> Proteolysis / Ubiquitin >> Proteolytic enzymes >> Serine protease >> Proprotein Convertases
Signal Transduction >> Metabolism >> Lipid metabolism
Stem Cells >> Neural Stem Cells >> Intracellular
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cholesterol Metabolism
Signal Transduction >> Metabolism >> Amino Acids
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Relevance
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PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. PCSK9 may function as a proprotein convertase. PCSK9 plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons. Mutations in PCSK9 gene have been associated with a third form of autosomal dominant familial hypercholesterolemia.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PCSK9 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PCSK9 antibody images, references, products related to ab52754 and other tools.
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PCSK9 antibody images:
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 Immunocytochemistry/ Immunofluorescence - PCSK9 antibody (ab52754)
ICC/IF image of ab52754 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52754, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to PCSK9
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PCSK9 antibody - more information
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Customer reviews (feedback) regarding PCSK9 antibody |
Customer FAQs regarding PCSK9 antibody |
Protocols for PCSK9 antibody |
Price and availability of products related to PCSK9 antibody |
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PCSK9 antibody - related products:
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PCSK9 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PCSK9 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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