Anti-PCSK9 antibody [mAbcam84041] (ab84041)

Overview

  • Product nameAnti-PCSK9 antibody [mAbcam84041]See all PCSK9 primary antibodies ...
  • Description
    Mouse monoclonal [mAbcam84041] to PCSK9
  • Tested applicationsWB, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Non Human Primates
  • Immunogen

    Recombinant fragment derived from within residues 100 - 200 of Human PCSK9.

  • Positive control
    • This antibody gave a positive signal in human heart and human kidney tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab84041 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionMay be implicated in the differentiation of cortical neurons and may play a role in cholesterol homeostasis.
  • Tissue specificityExpressed in neuro-epithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells.
  • Involvement in diseaseDefects in PCSK9 are the cause of familial hypercholesterolemia 3 (FH3) [MIM:603776]. FH3 inheritance is autosomal dominant.
  • Sequence similaritiesBelongs to the peptidase S8 family.
    Contains 1 peptidase S8 domain.
  • Post-translational
    modifications
    The soluble zymogen undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted enzyme.
  • Cellular localizationSecreted.
  • Target information above from: UniProt accession Q8NBP7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Convertase subtilisin/kexin type 9 preproprotein antibody
    • FH3 antibody
    • HCHOLA3 antibody
    • Hypercholesterolemia autosomal dominant 3 antibody
    • LDLCQ1 antibody
    • NARC 1 antibody
    • NARC-1 antibody
    • NARC1 antibody
    • Neural apoptosis regulated convertase 1 antibody
    • Neural apoptosis-regulated convertase 1 antibody
    • PC 9 antibody
    • PC9 antibody
    • PCSK 9 antibody
    • PCSK9 antibody
    • PCSK9_HUMAN antibody
    • Proprotein convertase 9 antibody
    • Proprotein convertase PC9 antibody
    • Proprotein convertase subtilisin/kexin type 9 antibody
    • PSEC0052 antibody
    • Subtilisin/kexin like protease PC9 antibody
    • Subtilisin/kexin-like protease PC9 antibody
    see all

Anti-PCSK9 antibody [mAbcam84041] images

  • All lanes : Anti-PCSK9 antibody [mAbcam84041] (ab84041) at 1/500 dilution

    Lane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
    Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Mouse IgM (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Additional bands at : 30 kDa,40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab84041 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab84041, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 and MCF7 cells at 5µg/ml.

References for Anti-PCSK9 antibody [mAbcam84041] (ab84041)

ab84041 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and your interest in our products.

The antibodies you are interested in are provided following different purification steps. The ab84041 is provides as concentrated culture supernatant, ab5000 is provides as IgG fr...

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Thank you for contacting us.

The Rabbit polyclonal to PCSK9 (ab125251;http://www.abcam.com/PCSK9-antibody-ab125251.html) which we spoke about earlier is our only product that has been tested in ELISA. It recognizes a region around aa100-300 ...

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