Recombinant
RabMAb

Anti-PD-L1 antibody [28-8] (Phycoerythrin) (ab209962)

Overview

  • Product name
    Anti-PD-L1 antibody [28-8] (Phycoerythrin)
    See all PD-L1 primary antibodies
  • Description
    Rabbit monoclonal [28-8] to PD-L1 (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human PD-L1 (extracellular). The immunogen contains the specific extracellular domain of huPD-L1 (Phe19-Thr239). See reference for more info - www.ncbi.nlm.nih.gov/pmc/articles/PMC4561627/
    Database link: Q9NZQ7

  • Positive control
    • Flow Cyt: CHO-PDL1 cells
  • General notes

    Anti-PD-L1 antibody [28-8] has been used as detector antibody in Human PD-L1 SimpleStep ELISA® kit (ab214565)

    Additional information on positive controls:

    Cell Lines:
    Positives: B-CPAP- high, ES-2- medium, HCC70 - low

    For primary negative control, isotype controlRabMAb negative control antibody (Ab172730) is recommended.

    For negative control sample, cell line COLO205 is recommended.

    For PD-L1 protein, see ab167713

    Recommended protocols:

    For recommended Flow Cytometry (Flow Cyt) protocol, please refer to the protocol book (line 1) in the protocol section and/or here (downloadable copy) 

    Alternative versions available:
    Anti-PD-L1 antibody [28-8] (ab205921) - Knockout validated

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab209962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

ab209478-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function
    Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
  • Tissue specificity
    Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
  • Sequence similarities
    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Cell membrane and Endomembrane system.
  • Information by UniProt
  • Database links
  • Alternative names
    • B7 H antibody
    • B7 H1 antibody
    • B7 homolog 1 antibody
    • B7-H1 antibody
    • B7H antibody
    • B7H1 antibody
    • CD 274 antibody
    • CD274 antibody
    • CD274 antigen antibody
    • CD274 molecule antibody
    • MGC142294 antibody
    • MGC142296 antibody
    • OTTHUMP00000021029 antibody
    • PD L1 antibody
    • PD-L1 antibody
    • PD1L1_HUMAN antibody
    • PDCD1 ligand 1 antibody
    • PDCD1L1 antibody
    • PDCD1LG1 antibody
    • PDL 1 antibody
    • PDL1 antibody
    • Programmed cell death 1 ligand 1 antibody
    • Programmed death ligand 1 antibody
    • RGD1566211 antibody
    see all

Images

  • Overlay histgram showing CHO (blue line) and CHO-PD-L1 transfected (red line) cells stained with ab209962.

    The cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209962, 1/100 dilution) for 30 min at 4ºC.

    Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/42 bandpass filter.

References

ab209962 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab209962.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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