Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Placenta)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
Permeabilization No
Specification Placenta
Blocking step Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative Formaldehyde

Other product details

Incubation time 32 minute(s) · Temperature: 37°C · Diluent: Ventana antibody diluent
Dilution 1/200

Secondary antibody

Name Non-Abcam antibody was used: Optiview detection system
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer

Additional data

Additional Notes Used Ventana Benchmark Ultra automated platform, with Optiview detection kit. Antigen retrieval was for 72 minutes at 100C. Photo shown is of placenta used for optimisation of antibody.

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Submitted Dec 04 2015