Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
Permeabilization
No
Specification
Placenta
Blocking step
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Fixative
Formaldehyde

Other product details

Incubation time
32 minute(s) · Temperature: 37°C · Diluent: Ventana antibody diluent
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Optiview detection system
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer

Additional data

Additional Notes
Used Ventana Benchmark Ultra automated platform, with Optiview detection kit. Antigen retrieval was for 72 minutes at 100C. Photo shown is of placenta used for optimisation of antibody.
Username

Abcam user community

Verified customer

Submitted Dec 04 2015

Sign up