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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH8.5
Ventana blocking agent as blocking agent for 4 minute(s) · Concentration: 100% · Temperature: 36°C
Other product details
32 minute(s) · Temperature: 37°C · Diluent: Ventana antibody diluent
Non-Abcam antibody was used: Optiview detection system
Host species: Goat
Conjugation: HRP polymer
Used Ventana Benchmark Ultra automated platform, with Optiview detection kit.
Antigen retrieval was for 72 minutes at 100C.
Photo shown is of placenta used for optimisation of antibody.
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Submitted Dec 04 2015