Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Tumor generated by a carcinogen (DMBA) or PANC02 m)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6
Permeabilization
No
Specification
Tumor generated by a carcinogen (DMBA) or PANC02 m
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Histofine Universal Immuno-peroxidase polymer anti
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
No reactivity with mouse PD-L1 is observed
Abcam response
We have not yet tested ab205921 in mouse in immunohistochemistry on paraffin sections. But for Western blot, we have tested this antibody using mouse samples, and it failed to detect PD-L1.
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Submitted Apr 15 2016

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