Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

Inconclusive
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Tumor generated by a carcinogen (DMBA) or PANC02 m)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6
Permeabilization No
Specification Tumor generated by a carcinogen (DMBA) or PANC02 m
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Paraformaldehyde

Other product details

Incubation time 18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution 1/100

Secondary antibody

Name Non-Abcam antibody was used: Histofine Universal Immuno-peroxidase polymer anti
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes No reactivity with mouse PD-L1 is observed
Abcam response We have not yet tested ab205921 in mouse in immunohistochemistry on paraffin sections. But for Western blot, we have tested this antibody using mouse samples, and it failed to detect PD-L1.
Username

Abcam user community

Verified customer

Submitted Apr 15 2016