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Abcam has not validated the combination of species/application used in this Abreview.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (Tumor generated by a carcinogen (DMBA) or PANC02 m)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6
Tumor generated by a carcinogen (DMBA) or PANC02 m
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 37°C
Other product details
18 hour(s) and 0 minute(s) · Temperature: 4°C
Non-Abcam antibody was used: Histofine Universal Immuno-peroxidase polymer anti
Host species: Goat
Conjugation: Horse Radish Peroxidase
No reactivity with mouse PD-L1 is observed
We have not yet tested ab205921 in mouse in immunohistochemistry on paraffin sections.
But for Western blot, we have tested this antibody using mouse samples, and it failed to detect PD-L1.
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Submitted Apr 15 2016