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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (NSQ-NSCLC)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EnVision FLEX TRS, Low pH
EnV FLEX Peroxidase-Blocking Reagent as blocking agent for 3 minute(s) · Concentration: 100%
Other product details
1 hour(s) and 0 minute(s) · Temperature: 25°C
This protocol used EnVision Flex Reagents for detection on the Dako Omnis automated stainer.
The Secondary Reagent used was the EnV FLEX + Rabbit Linker followed by the EnV FLEX/HRP Polymer.
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Submitted May 11 2016