Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-PD-L1 antibody [28-8]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (NSQ-NSCLC)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EnVision FLEX TRS, Low pH
Permeabilization
No
Specification
NSQ-NSCLC
Blocking step
EnV FLEX Peroxidase-Blocking Reagent as blocking agent for 3 minute(s) · Concentration: 100%
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/400

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
This protocol used EnVision Flex Reagents for detection on the Dako Omnis automated stainer. The Secondary Reagent used was the EnV FLEX + Rabbit Linker followed by the EnV FLEX/HRP Polymer.
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Submitted May 11 2016

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