Recombinant
RabMAb

Anti-PD1 antibody [EPR4877(2)] (ab137132)

Overview

  • Product name
    Anti-PD1 antibody [EPR4877(2)]
    See all PD1 primary antibodies
  • Description
    Rabbit monoclonal [EPR4877(2)] to PD1
  • Tested applications
    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PD1 aa 1-100.
    (Peptide available as ab173374)

  • Positive control
    • IHC-P: Human T cell lymphoma and Human tonsil tissues
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137132 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      Possible cell death inducer, in association with other factors.
    • Involvement in disease
      Genetic variation in PDCD1 is associated with susceptibility to systemic lupus erythematosus type 2 (SLEB2) [MIM:605218]. Systemic lupus erythematosus is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
    • Sequence similarities
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Developmental stage
      Induced at programmed cell death.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD279 antibody
      • CD279 antigen antibody
      • hPD 1 antibody
      • hPD l antibody
      • hPD-1 antibody
      • hSLE1 antibody
      • PD 1 antibody
      • PD-1 antibody
      • PD1 antibody
      • PDCD 1 antibody
      • PDCD1 antibody
      • PDCD1_HUMAN antibody
      • Programmed cell death 1 antibody
      • Programmed cell death 1 protein antibody
      • Programmed cell death protein 1 antibody
      • Protein PD 1 antibody
      • Protein PD-1 antibody
      • SLEB2 antibody
      • Systemic lupus erythematosus susceptibility 2 antibody
      see all

    Anti-PD1 antibody [EPR4877(2)] images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD1 with ab137132 at a dilution of 1/500. Heat mediated antigen retrieval was performed using EDTA antigen retrieval solution, and microwave treatment for 20 min at 20% power. Anti-Rabbit HRP polymer was used as secondary antibody. Opal tyramide amplification was performed using Opal 540 fluorophore. Counterstained with DAPI stain.

      Image scanned with Vectra 3.0 and analyzed via Inform software.

      This image was courteously provided by Dr. Houssein Abdul Sater, Georgia Cancer Center.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human T-cell lymphoma tissue labelling PD1 with unpurified ab137132 at a dilution of 1/250.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD1 with purified ab137132 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PD1 with unpurified ab137132 at a dilution of 1/250.

    • Immunohistochemical analysis using ab137132 showing negative staining in normal human brain tissue.

    • Immunohistochemical analysis using ab137132 showing negative staining in human skeletal muscle tissue.

    References for Anti-PD1 antibody [EPR4877(2)] (ab137132)

    This product has been referenced in:
    • Zhou ZH  et al. The prognostic value and pathobiological significance of Glasgow microenvironment score in gastric cancer. J Cancer Res Clin Oncol N/A:N/A (2017). IHC ; Human . Read more (PubMed: 28180998) »
    • Ring KL  et al. Potential immunotherapy targets in recurrent cervical cancer. Gynecol Oncol N/A:N/A (2017). IHC ; Human . Read more (PubMed: 28233576) »

    See all 14 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: sodium citrate buffer, pH 6
    Specification
    Tonsil
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 01 2016

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C, 30min
    Permeabilization
    No
    Specification
    tonsil
    Blocking step
    H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 25 2015

    I wanted to inform you that we have now added the blocking peptide for ab137132 Anti-PD1 antibody [EPR4877(2)]. This peptide, catalog number ab173374, may be found at: http://www.abcam.com/ab173374.html

    Thank you for contacting us.

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    I hope this information is helpful to you. Please do not hesit...

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