Anti-PDCD4 antibody [EPR3431] (ab80590)

Overview

• Product nameAnti-PDCD4 antibody [EPR3431]See all PDCD4 primary antibodies ...
• Description
  Rabbit monoclonal [EPR3431] to PDCD4
• Tested applicationsWB, IP, ICC, Flow Cyt, IHC-P more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  Synthetic peptide corresponding to residues near the N terminus of Human PDCD4

• Positive controlWB: Jurkat, HL60 + RA and HeLa cell lysates; IHC-P: Human tonsil tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Dissociation constant (Kd) Kd = 3.10 x 10^-11 M
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3431
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cell Biology
  • Apoptosis
  • Nucleus
  • Other

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Other

Applications

Target

• FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
• Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
• Sequence similaritiesBelongs to the PDCD4 family.
  Contains 2 MI domains.
• DomainBinds EIF4A1 via both MI domains.
• Post-translational
  modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
• Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.

Target information above from: UniProt accession Q53EL6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 27250 Human
  • Omim: 608610 Human
  • SwissProt: Q53EL6 Human
  • Unigene: 711490 Human

• Alternative names
  Death up-regulated gene protein antibodyDug antibodyH731 antibody

  Ma3 antibodyMGC33046 antibodyMGC33047 antibodyNeoplastic transformation inhibitor antibodyNeoplastic transformation inhibitor protein antibodyNuclear antigen H731 antibodyNuclear antigen H731 like antibodyNuclear antigen H731 like protein antibodyNuclear antigen H731-like antibodyOTTHUMP00000020483 antibodyPDCD 4 antibodyPdcd4 antibodyPDCD4_HUMAN antibodyProgrammed cell death 4 antibodyprogrammed cell death 4 (neoplastic transformation inhibitor) antibodyProgrammed cell death protein 4 antibodyProtein 197/15a antibodyProtein MA-3 antibodyRP11 348N5.4 antibodyTis antibodyTopoisomerase-inhibitor suppressed protein antibody

  see all

Anti-PDCD4 antibody [EPR3431] images

• 

  Western blot - PDCD4 antibody [EPR3431] (ab80590)
  All lanes : Anti-PDCD4 antibody [EPR3431] (ab80590) at 1/10000 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : HL60 + RA lysate
  Lane 3 : HeLa cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  goat anti-rabbit HRP at 1/2000 dilution
  
  Predicted band size : 52 kDa
  Observed band size : 52 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 antibody [EPR3431] (ab80590)
  Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using 1/100 ab80590.
• 

  Other-Anti-PDCD4 antibody [EPR3431](ab80590)
  Equilibrium disassociation constant (K_D)
  Learn more about K_D
  
  Click here to learn more about K_D