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Western blot - PDCD4 antibody [EPR3431] (ab80590)
![Western blot - PDCD4 antibody [EPR3431] (ab80590)](/ps/datasheet/images/80/ab80590/PDCD4-Primary-antibodies-ab80590-1.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 antibody [EPR3431] (ab80590)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 antibody [EPR3431] (ab80590)](/ps/datasheet/images/80/ab80590/PDCD4-Primary-antibodies-ab80590-2.jpg)
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Product Name
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PDCD4 antibody [EPR3431]
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See all PDCD4 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit monoclonal [EPR3431] to PDCD4
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Immunogen
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Synthetic peptide corresponding to residues near the N terminus of Human PDCD4
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Reacts with
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Hu
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Does not react with
(species key)
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Ms, Rat
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Tested applications
(see key)
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Flow Cyt, ICC, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Flow Cyt: 1/100.
ICC: 1/100 - 1/250.
IHC-P: 1/250 - 1/500. Antigen retrieval is recommended.
IP: 1/30.
WB: 1/20000 - 1/100000. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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WB: Jurkat, HL60 + RA and HeLa cell lysates;
IHC-P: Human tonsil tissue.
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Cellular localization
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Cytoplasmic and Nuclear.
Note: Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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PDCD4 encodes a protein localized to the nucleus in proliferating cells. Expression of PDCD4 is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Monoclonal
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Clone number
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EPR3431
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Isotype
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IgG
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Purity
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Tissue culture supernatant
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PDCD4 antibody [EPR3431] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PDCD4 antibody [EPR3431] images, references, products related to ab80590 and other tools.
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PDCD4 antibody [EPR3431] images:
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 Western blot - PDCD4 antibody [EPR3431] (ab80590)
All lanes : PDCD4 antibody [EPR3431] (ab80590) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HL60 + RA lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 52 kDa
Observed band size : 52 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 antibody [EPR3431] (ab80590)
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using 1/100 ab80590.
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Search PubMed (MEDLINE) for references to PDCD4
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PDCD4 antibody [EPR3431] - more information
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Customer reviews (feedback) regarding PDCD4 antibody [EPR3431] |
Customer FAQs regarding PDCD4 antibody [EPR3431] |
Protocols for PDCD4 antibody [EPR3431] |
Price and availability of products related to PDCD4 antibody [EPR3431] |
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PDCD4 antibody [EPR3431] - related products:
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PDCD4 antibody [EPR3431] - other tools:
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Contact Abcam with a Technical Enquiry about PDCD4 antibody [EPR3431] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - PDCD4 antibody [EPR3431] (ab80590)](/index.html?datasheet=80590#PDCD4-Primary-antibodies-ab80590-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 antibody [EPR3431] (ab80590)](/index.html?datasheet=80590#PDCD4-Primary-antibodies-ab80590-2.jpg)
