![Western blot - PDCD4 antibody [EPR3431] (ab80590) image](#PDCD4-Primary-antibodies-ab80590-1.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 antibody [EPR3431] (ab80590) image](#PDCD4-Primary-antibodies-ab80590-2.jpg)
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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-PDCD4 antibody [EPR3431]
See all PDCD4 products (15) ...
Description
Rabbit monoclonal [EPR3431] to PDCD4
Tested applications
WB, IP, ICC, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse, Rat
Immunogen
Synthetic peptide corresponding to residues near the N terminus of Human PDCD4
Positive control
WB: Jurkat, HL60 + RA and HeLa cell lysates; IHC-P: Human tonsil tissue.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR3431
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab80590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/20000 - 1/100000.Predicted molecular weight: 52 kDa.
IP
IP: 1/30IP: 1/30
ICC
ICC: 1/100 - 1/250.ICC: 1/100 - 1/250.
Flow Cyt
Flow Cyt: 1/100Flow Cyt: 1/100
IHC-P: 1/250 - 1/500.(Antigen retrieval is recommended.)
Target
Function
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Tissue specificity
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
Sequence similarities
Belongs to the PDCD4 family.
Contains 2 MI domains.
Domain
Binds EIF4A1 via both MI domains.
Post-translational
modifications
Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
Target information above from: UniProt accessionQ53EL6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
see all
Database links
- Entrez Gene: 27250 Human
- Omim: 608610 Human
- SwissProt: Q53EL6 Human
- Unigene: 711490 Human
Anti-PDCD4 antibody [EPR3431] images:
Western blot - PDCD4 antibody [EPR3431] (ab80590)
![Western blot - PDCD4 antibody [EPR3431] (ab80590)](/ps/datasheet/images/80/ab80590/PDCD4-Primary-antibodies-ab80590-1.jpg)
All lanes : Anti-PDCD4 antibody [EPR3431] (ab80590) at 1/10000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HL60 + RA lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Predicted band size : 52 kDa
Observed band size : 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 antibody [EPR3431] (ab80590)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 antibody [EPR3431] (ab80590)](/ps/datasheet/images/80/ab80590/PDCD4-Primary-antibodies-ab80590-2.jpg)
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using 1/100 ab80590.
References for Anti-PDCD4 antibody [EPR3431] (ab80590)
This product has been referenced in:
- Liu Xet al. MicroRNA-499-5p promotes cellular invasion and tumor metastasis in colorectal cancer by targeting FOXO4 and PDCD4. Carcinogenesis 32:1798-805 (2011). WB; Human.Read more (PubMed: 21934092) »
See 1 publication for this product
Publishing research using ab80590? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"