Overview

  • Product nameAnti-PDCD4 antibody [EPR3432]
    See all PDCD4 primary antibodies
  • Description
    Rabbit monoclonal [EPR3432] to PDCD4
  • Tested applicationsWB, IHC-P, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human

    Does not react with

    Rat
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human PDCD4

  • Positive control
    • Jurkat and HeLa cell lysates; human breast carcinoma tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab79405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC
Flow Cyt
  • Application notesFlow Cyt: 1/50.
    ICC: 1/250 - 1/500.
    IHC-P: 1/1000 - 1/2000. Antigen retrieval is recommended.
    WB: 1/5000 - 1/10000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

    Is unsuitable for IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
    • Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
    • Sequence similaritiesBelongs to the PDCD4 family.
      Contains 2 MI domains.
    • DomainBinds EIF4A1 via both MI domains.
    • Post-translational
      modifications
      Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
    • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
    • Information by UniProt
    • Database links
    • Alternative names
      • Death up-regulated gene protein antibody
      • Dug antibody
      • H731 antibody
      • Ma3 antibody
      • MGC33046 antibody
      • MGC33047 antibody
      • Neoplastic transformation inhibitor antibody
      • Neoplastic transformation inhibitor protein antibody
      • Nuclear antigen H731 antibody
      • Nuclear antigen H731 like antibody
      • Nuclear antigen H731 like protein antibody
      • Nuclear antigen H731-like antibody
      • OTTHUMP00000020483 antibody
      • PDCD 4 antibody
      • Pdcd4 antibody
      • PDCD4_HUMAN antibody
      • Programmed cell death 4 antibody
      • programmed cell death 4 (neoplastic transformation inhibitor) antibody
      • Programmed cell death protein 4 antibody
      • Protein 197/15a antibody
      • Protein MA-3 antibody
      • RP11 348N5.4 antibody
      • Tis antibody
      • Topoisomerase-inhibitor suppressed protein antibody
      see all

    Anti-PDCD4 antibody [EPR3432] images

    • All lanes : Anti-PDCD4 antibody [EPR3432] (ab79405) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 52 kDa
      Observed band size : 52 kDa
      Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
    • ab79405 at 1/1000 dilution staining PDCD4 in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue.

    References for Anti-PDCD4 antibody [EPR3432] (ab79405)

    ab79405 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab79405.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"