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Overview

  • Product nameAnti-PDCD4 antibodySee all PDCD4 primary antibodies ...
  • Description
    Rabbit polyclonal to PDCD4
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog
  • Immunogen

    A region within synthetic peptide: MTKYPDNLSD SLFSGDEENA GTEEIKNEIN GNWISASSIN EARINAKAKR, corresponding to N terminal amino acids 1-50 of Human PDCD4

  • Positive controlPlacenta lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab32831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: 1/62500.
WB WB: Use a concentration of 0.5 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • Sequence similaritiesBelongs to the PDCD4 family.
    Contains 2 MI domains.
  • DomainBinds EIF4A1 via both MI domains.
  • Post-translational
    modifications
    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Target information above from: UniProt accession Q53EL6 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
      Death up-regulated gene protein antibodyDug antibodyH731 antibody
      Ma3 antibodyMGC33046 antibodyMGC33047 antibodyNeoplastic transformation inhibitor antibodyNeoplastic transformation inhibitor protein antibodyNuclear antigen H731 antibodyNuclear antigen H731 like antibodyNuclear antigen H731 like protein antibodyNuclear antigen H731-like antibodyPDCD 4 antibodyPdcd4 antibodyPDCD4_HUMAN antibodyprogrammed cell death 4 (neoplastic transformation inhibitor) antibodyProgrammed cell death 4 antibodyProgrammed cell death protein 4 antibodyProtein 197/15a antibodyProtein MA-3 antibodyRP11-348N5.4 antibodyTis antibodyTopoisomerase-inhibitor suppressed protein antibody
    see all

Anti-PDCD4 antibody images

  • Anti-PDCD4 antibody (ab32831) at 0.5 µg/ml + placental lysate at 1 mg/ml

    Secondary
    HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

    Predicted band size : 52 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PDCD4 antibody (ab32831)

This product has been referenced in:
  • Pan Q  et al. microRNA 21: response to hormonal therapies and regulatory function in leiomyoma, transformed leiomyoma and leiomyosarcoma cells. Mol Hum Reprod 16:215-27 (2010). WB ; Human . Read more (PubMed: 19906824) »
  • Folini M  et al. miR-21: an oncomir on strike in prostate cancer. Mol Cancer 9:12 (2010). WB ; Human . Read more (PubMed: 20092645) »

See all 2 Publications for this product

Product Wall

Displaying 1 - 1 of 1 results for Abreviews and Q&A

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (C2C12 cells)
Loading amount 45 µg
Specification C2C12 cells
Treatment AICAR and dexamethasone
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Sep 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"