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Western blot - PDCD4 antibody (ab32831)
(enlarge)
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Product Name
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PDCD4 antibody
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See all PDCD4 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PDCD4
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Immunogen
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A region within synthetic peptide: MTKYPDNLSD SLFSGDEENA GTEEIKNEIN GNWISASSIN EARINAKAKR, corresponding to N terminal amino acids 1-50 of Human PDCD4
BLAST 'MTKYPDNLSD SLFSGDEENA GTEEIKNEIN GNWISASSIN EARINAKAKR ' with  or 
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/62500.
WB: Use at a concentration of 0.5 µg/ml. Predicted molecular weight: 52 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Placenta lysate.
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Cellular localization
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Cytoplasmic and Nuclear.
Note: Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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PDCD4 encodes a protein localized to the nucleus in proliferating cells. Expression of PDCD4 is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: 2% Sucrose, PBS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PDCD4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PDCD4 antibody images, references, products related to ab32831 and other tools.
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PDCD4 antibody images:
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 Western blot - PDCD4 antibody (ab32831)
PDCD4 antibody (ab32831) at 0.5 µg/ml + placental lysate at 1 mg/ml
Secondary
HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000
Predicted band size : 52 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.
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Search PubMed (MEDLINE) for references to PDCD4
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PDCD4 antibody - more information
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Customer reviews (feedback) regarding PDCD4 antibody |
Customer FAQs regarding PDCD4 antibody |
Protocols for PDCD4 antibody |
Price and availability of products related to PDCD4 antibody |
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PDCD4 antibody - related products:
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PDCD4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PDCD4 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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