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Anti-PDCD4 antibody (ab32831)

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Overview

Product name

Anti-PDCD4 antibody
See all PDCD4 products (15) ...

Description

Rabbit polyclonal to PDCD4

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Dog

Immunogen

A region within synthetic peptide: MTKYPDNLSD SLFSGDEENA GTEEIKNEIN GNWISASSIN EARINAKAKR, corresponding to N terminal amino acids 1-50 of Human PDCD4

MTKYPDNLSD SLFSGDEENA GTEEIKNEIN GNWISASSIN EARINAKAKR

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Placenta lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

  • Western blot - PDCD4 antibody (ab32831)Western blot - PDCD4 antibody (ab32831) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.

Tissue specificity

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.

Sequence similarities

Belongs to the PDCD4 family.
Contains 2 MI domains.

Domain

Binds EIF4A1 via both MI domains.

Post-translational
modifications

Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.

Cellular localization

Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.

Target information above from: UniProt accessionQ53EL6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Death up-regulated gene protein antibody
  • Dug antibody
  • H731 antibody
  • Ma3 antibody
  • MGC33046 antibody
  • MGC33047 antibody
  • Neoplastic transformation inhibitor antibody
  • Neoplastic transformation inhibitor protein antibody
  • Nuclear antigen H731 antibody
  • Nuclear antigen H731 like antibody
  • Nuclear antigen H731 like protein antibody
  • Nuclear antigen H731-like antibody
  • PDCD 4 antibody
  • Pdcd4 antibody
  • PDCD4_HUMAN antibody
  • programmed cell death 4 (neoplastic transformation inhibitor) antibody
  • Programmed cell death 4 antibody
  • Programmed cell death protein 4 antibody
  • Protein 197/15a antibody
  • Protein MA-3 antibody
  • RP11-348N5.4 antibody
  • Tis antibody
  • Topoisomerase-inhibitor suppressed protein antibody
see all

Database links

see all

Anti-PDCD4 antibody images:

  Western blot - PDCD4 antibody (ab32831)

Western blot - PDCD4 antibody (ab32831)

Anti-PDCD4 antibody (ab32831) at 0.5 µg/ml + placental lysate at 1 mg/ml

Secondary
HRP conjugated anti-Rabbit IgG 1: 50,000 - 100,000

Predicted band size : 52 kDa
Observed band size : 50 kDa (why is the actual band size different from the predicted?)
Additional bands at : 20 kDa. We are unsure as to the identity of these extra bands.

References for Anti-PDCD4 antibody (ab32831)

This product has been referenced in:

  • Pan Qet al. microRNA 21: response to hormonal therapies and regulatory function in leiomyoma, transformed leiomyoma and leiomyosarcoma cells. Mol Hum Reprod 16:215-27 (2010). WB; Human.Read more (PubMed: 19906824) »
  • Folini Met al. miR-21: an oncomir on strike in prostate cancer. Mol Cancer 9:12 (2010). WB; Human.Read more (PubMed: 20092645) »

See all 2 publications for this product

Publishing research using ab32831? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"