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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-PDCD4 antibody
See all PDCD4 products (15) ...
Description
Rabbit polyclonal to PDCD4
Tested applications
IP, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Orangutan
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human PDCD4.
(Peptide available as ab45261.)
Positive control
ab45124 gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; SHSY-5Y (data not shown)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab45124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. (PubMed: 21163940)
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlDetects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Target
Function
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Tissue specificity
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
Sequence similarities
Belongs to the PDCD4 family.
Contains 2 MI domains.
Domain
Binds EIF4A1 via both MI domains.
Post-translational
modifications
Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
Target information above from: UniProt accessionQ53EL6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
see all
Database links
- Entrez Gene: 374191 Chicken
- Entrez Gene: 506724 Cow
- Entrez Gene: 27250 Human
- Entrez Gene: 18569 Mouse
- Entrez Gene: 64031 Rat
- Omim: 608610 Human
- SwissProt: Q98TX3 Chicken
- SwissProt: Q53EL6 Human
see all
Anti-PDCD4 antibody images:
Western blot - PDCD4 antibody (ab45124)
All lanes : Anti-PDCD4 antibody (ab45124) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 52 kDa
Additional bands at : 45 kDa,60 kDa. We are unsure as to the identity of these extra bands.
The PDCD4 protein has a number of possible phosphorylated residues (SwissProt data) and isoforms, which may explain the banding pattern observed at the expected molecular weight. An additional band at ~60 kDa has been observed in some batches of PDCD4.
Immunocytochemistry/ Immunofluorescence - PDCD4 antibody (ab45124)
ICC/IF image of ab45124 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab45124, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
References for Anti-PDCD4 antibody (ab45124)
This product has been referenced in:
- Soler-López Met al. Interactome mapping suggests new mechanistic details underlying Alzheimer's disease. Genome Res 21:364-76 (2011). WB, IP; Human.Read more (PubMed: 21163940) »
See 1 publication for this product
Publishing research using ab45124? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"