Anti-PDCD4 antibody (ab45124)

Overview

• Product nameAnti-PDCD4 antibodySee all PDCD4 primary antibodies ...
• Description
  Rabbit polyclonal to PDCD4
• Tested applicationsIP, IHC-P, ICC/IF, WB more details
• Species reactivity
  Reacts with: Mouse, Human
  Predicted to work with: Rat, Chicken, Cow, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human PDCD4.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab45261.)

• Positive controlab45124 gave a positive signal in the following tissue and whole cell lysates in WB: HeLa, Jurkat, HEK293, Mouse Thymus, Mouse Liver, MEF1, SHSY-5Y (data not shown); and in IF: HeLa IHC-P: Mouse liver FFPE tissue sections.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cell Biology
  • Apoptosis
  • Nucleus
  • Other

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Other

Applications

Target

• FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
• Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
• Sequence similaritiesBelongs to the PDCD4 family.
  Contains 2 MI domains.
• DomainBinds EIF4A1 via both MI domains.
• Post-translational
  modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
• Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.

Target information above from: UniProt accession Q53EL6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 374191 Chicken
  • Entrez Gene: 506724 Cow
  • Entrez Gene: 27250 Human
  • Entrez Gene: 18569 Mouse
  • Entrez Gene: 64031 Rat
  • Omim: 608610 Human
  • SwissProt: Q98TX3 Chicken
  • SwissProt: Q53EL6 Human

  • SwissProt: Q61823 Mouse
  • SwissProt: Q5R8S3 Orangutan
  • SwissProt: Q9JID1 Rat
  • Unigene: 711490 Human
  • Unigene: 1605 Mouse
  • Unigene: 375091 Mouse
  • Unigene: 206228 Rat

  see all

• Alternative names
  Death up-regulated gene protein antibodyDug antibodyH731 antibody

  Ma3 antibodyMGC33046 antibodyMGC33047 antibodyNeoplastic transformation inhibitor antibodyNeoplastic transformation inhibitor protein antibodyNuclear antigen H731 antibodyNuclear antigen H731 like antibodyNuclear antigen H731 like protein antibodyNuclear antigen H731-like antibodyPDCD 4 antibodyPdcd4 antibodyPDCD4_HUMAN antibodyProgrammed cell death 4 antibodyprogrammed cell death 4 (neoplastic transformation inhibitor) antibodyProgrammed cell death protein 4 antibodyProtein 197/15a antibodyProtein MA-3 antibodyTis antibodyTopoisomerase-inhibitor suppressed protein antibody

  see all

Anti-PDCD4 antibody images

• 

  Western blot - PDCD4 antibody (ab45124)
  All lanes : Anti-PDCD4 antibody (ab45124) at 1 µg/ml
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 52 kDa
  Observed band size : 52 kDa
  Additional bands at : 45 kDa,60 kDa. We are unsure as to the identity of these extra bands.
• 

  Western blot - Anti-PDCD4 antibody (ab45124)
  All lanes : Anti-PDCD4 antibody (ab45124) at 1 µg/ml
  
  Lane 1 : Thymus (Mouse) Tissue Lysate
  Lane 2 : Liver (Mouse) Tissue Lysate
  Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 52 kDa
  Observed band size : 52 kDa
  Additional bands at : 45 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 2 minutes
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDCD4 antibody (ab45124)
  IHC image of PDCD4 staining in mouse liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45124, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
• 

  Immunocytochemistry/ Immunofluorescence - PDCD4 antibody (ab45124)
  ICC/IF image of ab45124 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab45124, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).