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Western blot - PDCD4 antibody (ab45124)
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Immunocytochemistry/ Immunofluorescence - PDCD4 antibody (ab45124)
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Product Name
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PDCD4 antibody
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See all PDCD4 antibodies (11)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PDCD4
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human PDCD4. (Peptide available as ab45261.)
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Chk, Cow, Orngt
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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ab45124 gave a positive signal in the following whole cell lysates:
HeLa; Jurkat; HEK293; SHSY-5Y (data not shown)
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Cellular localization
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Cytoplasmic and Nuclear.
Note: Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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PDCD4 encodes a protein localized to the nucleus in proliferating cells. Expression of PDCD4 is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PDCD4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PDCD4 antibody images, references, products related to ab45124 and other tools.
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PDCD4 antibody images:
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 Western blot - PDCD4 antibody (ab45124)
All lanes : PDCD4 antibody (ab45124) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 52 kDa
Observed band size : 52 kDa
Additional bands at : 45 kDa,60 kDa. We are unsure as to the identity of these extra bands.
The PDCD4 protein has a number of possible phosphorylated residues (SwissProt data) and isoforms, which may explain the banding pattern observed at the expected molecular weight. An additional band at ~60 kDa has been observed in some batches of PDCD4.
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Immunocytochemistry/ Immunofluorescence - PDCD4 antibody (ab45124)
ICC/IF image of ab45124 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab45124, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Search PubMed (MEDLINE) for references to PDCD4
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PDCD4 antibody - more information
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Customer reviews (feedback) regarding PDCD4 antibody |
Customer FAQs regarding PDCD4 antibody |
Protocols for PDCD4 antibody |
Price and availability of products related to PDCD4 antibody |
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PDCD4 antibody - related products:
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PDCD4 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PDCD4 antibody |
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