Overview

  • Product nameAnti-PDCD4 antibodySee all PDCD4 primary antibodies ...
  • Description
    Rabbit polyclonal to PDCD4
  • Tested applicationsIP, IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human PDCD4.

    (Peptide available as ab45261.)

  • Positive control
    • ab45124 gave a positive signal in the following tissue and whole cell lysates in WB: HeLa, Jurkat, HEK293, Mouse Thymus, Mouse Liver, MEF1, SHSY-5Y (data not shown); and in IF: HeLa IHC-P: Mouse liver FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab45124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. PubMed: 21163940
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human PDCD4 peptide (ab45261) or Human PDCD4 peptide (ab45262).

Target

  • FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • Sequence similaritiesBelongs to the PDCD4 family.
    Contains 2 MI domains.
  • DomainBinds EIF4A1 via both MI domains.
  • Post-translational
    modifications
    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

Anti-PDCD4 antibody images

  • All lanes : Anti-PDCD4 antibody (ab45124) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HEK293 Human embryonic kidney cell line Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 45 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-PDCD4 antibody (ab45124) at 1 µg/ml

    Lane 1 : Thymus (Mouse) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 45 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • IHC image of PDCD4 staining in mouse liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45124, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • ICC/IF image of ab45124 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab45124, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

References for Anti-PDCD4 antibody (ab45124)

This product has been referenced in:
  • Soler-López M  et al. Interactome mapping suggests new mechanistic details underlying Alzheimer's disease. Genome Res 21:364-76 (2011). WB, IP ; Human . Read more (PubMed: 21163940) »

See 1 Publication for this product

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