Anti-PDCD4 antibody (ab51495)
Key features and details
- Rabbit polyclonal to PDCD4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-PDCD4 antibody
See all PDCD4 primary antibodies -
Description
Rabbit polyclonal to PDCD4 -
Host species
Rabbit -
Specificity
ab51495 reacts with PDCD4 protein that is either phosphorylated or non phosphorylated at Ser 457. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis -
Immunogen
A synthetic peptide corresponding to amino acids near the carboxyl terminus of human PDCD4 protein
-
Positive control
- WB: 293 HEK cells, Mouse N2A cell Lysate. IHC-P: Mouse colon tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab51495 was affinity purified from monospecific antiserum by immunoaffinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (4) |
1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
|
IHC-P |
1/100 - 1/300.
|
Notes |
---|
WB
1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
IHC-P
1/100 - 1/300. |
Target
-
Function
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA. -
Tissue specificity
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma. -
Sequence similarities
Belongs to the PDCD4 family.
Contains 2 MI domains. -
Domain
Binds EIF4A1 via both MI domains. -
Post-translational
modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum. - Information by UniProt
-
Database links
- Entrez Gene: 27250 Human
- Entrez Gene: 18569 Mouse
- Entrez Gene: 64031 Rat
- Omim: 608610 Human
- SwissProt: Q53EL6 Human
- SwissProt: Q61823 Mouse
- SwissProt: Q9JID1 Rat
- Unigene: 711490 Human
see all -
Alternative names
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
see all
Images
-
All lanes : Anti-PDCD4 antibody (ab51495) at 1/5000 dilution
Lane 1 : recombinant PDCD4
Lane 2 : 293 HEK cells treated with TPA and MG132.
Predicted band size: 52 kDa
Observed band size: 52 kDa -
ab51495, at 1/100 dilution, staining human PDCD4 in paraformaldehyde fixed (4%), paraffin embedded mouse colon tissue by Immunohistochemistry.
-
All lanes : Anti-PDCD4 antibody (ab51495) at 1/1000 dilution
Lane 1 : Human HEK cell Lysate at 30 ug/ml
Lane 2 : Mouse N2A cell Lysate at 30 ug/ml
Secondary
All lanes : Li-Cor IRDye®800CW-conjugated Goat anti rabbit
Predicted band size: 52 kDa
13% SDS-Page gel used, Blocking done for 1 hour at RT, Primary Incubation overnight at 4°C.
Protocols
Datasheets and documents
-
Datasheet download
References (37)
ab51495 has been referenced in 37 publications.
- Yao M et al. Exosomal miR-21 secreted by IL-1ß-primed-mesenchymal stem cells induces macrophage M2 polarization and ameliorates sepsis. Life Sci 264:118658 (2021). PubMed: 33115604
- Wen C et al. Circ_0003266 sponges miR-503-5p to suppress colorectal cancer progression via regulating PDCD4 expression. BMC Cancer 21:284 (2021). PubMed: 33726686
- Zhao SF et al. Long non-coding RNA KCNQ1OT1 increases the expression of PDCD4 by targeting miR-181a-5p, contributing to cardiomyocyte apoptosis in diabetic cardiomyopathy. Acta Diabetol 58:1251-1267 (2021). PubMed: 33907874
- Hiraku Y et al. MicroRNA expression in lung tissues of asbestos-exposed mice: Upregulation of miR-21 and downregulation of tumor suppressor genes Pdcd4 and Reck. J Occup Health 63:e12282 (2021). PubMed: 34679210
- Guan K et al. Hsa_circ_0008259 modulates miR-21-5p and PDCD4 expression to restrain osteosarcoma progression. Aging (Albany NY) 13:25484-25495 (2021). PubMed: 34905503