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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-PDCD4 antibody
See all PDCD4 products (15) ...
Description
Rabbit polyclonal to PDCD4
Specificity
ab51495 reacts with PDCD4 protein that is either phosphorylated or non phosphorylated at Ser 457.
Tested applications
WB, ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Xenopus laevis
Immunogen
A synthetic peptide corresponding to amino acids near the carboxyl terminus of human PDCD4 protein
Positive control
WB: 293 HEK cells treated with TPA and MG132. IHC-P: Recombinant PDCD4 and mouse colon tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab51495 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab51495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000.Predicted molecular weight: 52 kDa.
ELISA: 1/10000 - 1/50000.
IHC-P: 1/100 - 1/300.
Target
Function
Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
Tissue specificity
Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
Sequence similarities
Belongs to the PDCD4 family.
Contains 2 MI domains.
Domain
Binds EIF4A1 via both MI domains.
Post-translational
modifications
Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
Target information above from: UniProt accessionQ53EL6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Death up-regulated gene protein antibody
- Dug antibody
- H731 antibody
see all
Database links
- Entrez Gene: 27250 Human
- Entrez Gene: 18569 Mouse
- Entrez Gene: 64031 Rat
- Entrez Gene: 380222 Xenopus laevis
- Omim: 608610 Human
- SwissProt: Q53EL6 Human
- SwissProt: Q61823 Mouse
- SwissProt: Q9JID1 Rat
see all
Anti-PDCD4 antibody images:
Western blot - PDCD4 antibody (ab51495)
All lanes : Anti-PDCD4 antibody (ab51495) at 1/5000 dilution
Lane 1 : recombinant PDCD4
Lane 2 : 293 HEK cells treated with TPA and MG132.
Predicted band size : 52 kDa
Observed band size : 52 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 antibody (ab51495)
ab51495, at 1/100 dilution, staining human PDCD4 in paraformaldehyde fixed (4%), paraffin embedded mouse colon tissue by Immunohistochemistry.
References for Anti-PDCD4 antibody (ab51495)
This product has been referenced in:
- Deftereos Get al. Expression of Mir-21 and Mir-143 in Cervical Specimens Ranging from Histologically Normal through to Invasive Cervical Cancer. PLoS One 6:e28423 (2011). IHC-P.Read more (PubMed: 22194833) »
- Haraguchi Tet al. Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells. Nucleic Acids Res 37:e43 (2009). WB.Read more (PubMed: 19223327) »
See all 5 publications for this product
Publishing research using ab51495? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"