Overview

  • Product nameAnti-PDCD4 antibodySee all PDCD4 primary antibodies ...
  • Description
    Rabbit polyclonal to PDCD4
  • Specificityab51495 reacts with PDCD4 protein that is either phosphorylated or non phosphorylated at Ser 457.
  • Tested applicationsIP, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis
  • Immunogen

    A synthetic peptide corresponding to amino acids near the carboxyl terminus of human PDCD4 protein

  • Positive control
    • WB: 293 HEK cells treated with TPA and MG132. IHC-P: Recombinant PDCD4 and mouse colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab51495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
ELISA 1/10000 - 1/50000.
IHC-P 1/100 - 1/300.

Target

  • FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • Sequence similaritiesBelongs to the PDCD4 family.
    Contains 2 MI domains.
  • DomainBinds EIF4A1 via both MI domains.
  • Post-translational
    modifications
    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • RP11 348N5.4 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

Anti-PDCD4 antibody images

  • All lanes : Anti-PDCD4 antibody (ab51495) at 1/5000 dilution

    Lane 1 : recombinant PDCD4
    Lane 2 : 293 HEK cells treated with TPA and MG132.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • ab51495, at 1/100 dilution, staining human PDCD4 in paraformaldehyde fixed (4%), paraffin embedded mouse colon tissue by Immunohistochemistry.
  • All lanes : Anti-PDCD4 antibody (ab51495) at 1/1000 dilution

    Lane 1 : Human HEK cell Lysate at 30 ug/ml
    Lane 2 : Mouse N2A cell Lysate at 30 ug/ml

    Secondary
    Li-Cor IRDye®800CW-conjugated Goat anti rabbit

    Predicted band size : 52 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-PDCD4 antibody (ab51495)

This product has been referenced in:
  • Fay MM  et al. Enhanced Arginine Methylation of Programmed Cell Death 4 Protein during Nutrient Deprivation Promotes Tumor Cell Viability. J Biol Chem 289:17541-17552 (2014). WB, ICC/IF ; Human . Read more (PubMed: 24764298) »
  • Muppala S  et al. CD24 induces expression of the oncomir miR-21 via Src, and CD24 and Src are both post-transcriptionally downregulated by the tumor suppressor miR-34a. PLoS One 8:e59563 (2013). Human . Read more (PubMed: 23533633) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Neuroblastoma cell line N2A)
Loading amount 30 µg
Specification Mouse Neuroblastoma cell line N2A
Gel Running Conditions Reduced Denaturing (13%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Verified customer

Submitted Jul 23 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (SW480)
Total protein in input 30 µg
Specification SW480
Treatment siRNA 40uM
Immuno-precipitation step Protein G
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Submitted Jun 20 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLA)
Loading amount 30 µg
Specification HeLA
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Oct 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 20 µg
Specification HEK293
Gel Running Conditions Non-reduced Non-Denaturing (Native) (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
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Submitted Oct 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 10 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted May 01 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"