Overview
- Product nameAnti-PDCD4 antibodySee all PDCD4 primary antibodies ...
- DescriptionRabbit polyclonal to PDCD4
- Specificityab51495 reacts with PDCD4 protein that is either phosphorylated or non phosphorylated at Ser 457.
- Tested applicationsIP, WB, ELISA, IHC-P more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis - Immunogen
A synthetic peptide corresponding to amino acids near the carboxyl terminus of human PDCD4 protein
- Positive controlWB: 293 HEK cells treated with TPA and MG132. IHC-P: Recombinant PDCD4 and mouse colon tissue.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab51495 was affinity purified from monospecific antiserum by immunoaffinity chromatography.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab51495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IP | IP: Use at an assay dependent concentration. |
| WB | WB: 1/1000 - 1/10000. Predicted molecular weight: 52 kDa. |
| ELISA | ELISA: 1/10000 - 1/50000. |
| IHC-P | IHC-P: 1/100 - 1/300. |
Target
- FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
- Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
- Sequence similaritiesBelongs to the PDCD4 family.
Contains 2 MI domains. - DomainBinds EIF4A1 via both MI domains.
- Post-translational
modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation. - Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
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Database links
- Entrez Gene: 27250 Human
- Entrez Gene: 18569 Mouse
- Entrez Gene: 64031 Rat
- Omim: 608610 Human
- SwissProt: Q53EL6 Human
- SwissProt: Q61823 Mouse
- SwissProt: Q9JID1 Rat
- Unigene: 711490 Human
- Unigene: 1605 Mouse
- Unigene: 375091 Mouse
- Unigene: 206228 Rat
see all
Target information above from: UniProt accession
Q53EL6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Death up-regulated gene protein antibodyDug antibodyH731 antibody
- Ma3 antibodyMGC33046 antibodyMGC33047 antibodyNeoplastic transformation inhibitor antibodyNeoplastic transformation inhibitor protein antibodyNuclear antigen H731 antibodyNuclear antigen H731 like antibodyNuclear antigen H731 like protein antibodyNuclear antigen H731-like antibodyPDCD 4 antibodyPdcd4 antibodyPDCD4_HUMAN antibodyProgrammed cell death 4 antibodyprogrammed cell death 4 (neoplastic transformation inhibitor) antibodyProgrammed cell death protein 4 antibodyProtein 197/15a antibodyProtein MA-3 antibodyRP11 348N5.4 antibodyTis antibodyTopoisomerase-inhibitor suppressed protein antibody
see all
Anti-PDCD4 antibody images
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Western blot - PDCD4 antibody (ab51495)All lanes : Anti-PDCD4 antibody (ab51495) at 1/5000 dilution
Lane 1 : recombinant PDCD4
Lane 2 : 293 HEK cells treated with TPA and MG132.
Predicted band size : 52 kDa
Observed band size : 52 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 antibody (ab51495)ab51495, at 1/100 dilution, staining human PDCD4 in paraformaldehyde fixed (4%), paraffin embedded mouse colon tissue by Immunohistochemistry. -
Western blot - Anti-PDCD4 antibody (ab51495)All lanes : Anti-PDCD4 antibody (ab51495) at 1/1000 dilution
Lane 1 : Human HEK cell Lysate at 30 ug/ml
Lane 2 : Mouse N2A cell Lysate at 30 ug/ml
Secondary
Li-Cor IRDye®800CW-conjugated Goat anti rabbit
Predicted band size : 52 kDaThis image is courtesy of an anonymous Abreview
References for Anti-PDCD4 antibody (ab51495)
This product has been referenced in:
- Deftereos G et al. Expression of Mir-21 and Mir-143 in Cervical Specimens Ranging from Histologically Normal through to Invasive Cervical Cancer. PLoS One 6:e28423 (2011). IHC-P . Read more (PubMed: 22194833) »
- Haraguchi T et al. Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells. Nucleic Acids Res 37:e43 (2009). WB . Read more (PubMed: 19223327) »
