PDCD4 antibody (ab51495)

Product Name 

PDCD4 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to PDCD4

Immunogen 

A synthetic peptide corresponding to amino acids near the carboxyl terminus of human PDCD4 protein

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Xl
(Due to sequence homology)

Specificity 

ab51495 reacts with PDCD4 protein that is either phosphorylated or non phosphorylated at Ser 457.

Tested applications 
(see key)

ELISA, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ELISA: 1/10000 - 1/50000.
IHC-P: 1/100 - 1/300. Tissue sections were deparaffinized and treated by trypsinization before staining.
WB: 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

WB: 293 HEK cells treated with TPA and MG132.
IHC-P: Recombinant PDCD4 and mouse colon tissue.

Cellular localization 

Cytoplasmic and Nuclear.
Note: Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions.

Research areas 

Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

Relevance 

PDCD4 encodes a protein localized to the nucleus in proliferating cells. Expression of PDCD4 is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

ab51495 was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Form 

Liquid

Concentration 

Batch dependent within range: 1.06 - 1.06 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for PDCD4 antibody images, references, products related to ab51495 and other tools.

PDCD4 antibody images:

References for PDCD4 antibody (ab51495)

  This product has been used in: (two most recent references)

Haraguchi T et al. Vectors expressing efficient RNA decoys achieve the long-term suppression of specific microRNA activity in mammalian cells. Nucleic Acids Res 37:e43 (2009). WB. PubMed: 19223327

Yamamichi N et al. Locked nucleic acid in situ hybridization analysis of miR-21 expression during colorectal cancer development. Clin Cancer Res 15:4009-16 (2009). IHC-P; Human. PubMed: 19509156

See all 4 publication references for this product.


If you publish research using ab51495 please let us know so that we can cite the reference on this datasheet.

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