Overview

  • Product nameAnti-PDCD4 antibodySee all PDCD4 primary antibodies ...
  • Description
    Rabbit polyclonal to PDCD4
  • Tested applicationsWB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human PDCD4 around the phosphorylation site of serine 457

  • Positive control
    • 293 cells; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab63359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • Sequence similaritiesBelongs to the PDCD4 family.
    Contains 2 MI domains.
  • DomainBinds EIF4A1 via both MI domains.
  • Post-translational
    modifications
    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • RP11 348N5.4 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

Anti-PDCD4 antibody images

  • All lanes : Anti-PDCD4 antibody (ab63359) at 1/500 dilution

    Lane 1 : extracts from 293 cells
    Lane 2 : extracts from 293 cells, with immunizing peptide


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
    Additional bands at : 56 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of PDCD4 expression in HeLa cells, using 1/500 ab63359. Left: untreated cells. Right: cells treated with immunising peptide.

References for Anti-PDCD4 antibody (ab63359)

ab63359 has not yet been referenced specifically in any publications.

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