Anti-PDCD4 antibody (ab63359)
- Product nameAnti-PDCD4 antibodySee all PDCD4 primary antibodies ...
- DescriptionRabbit polyclonal to PDCD4
- Tested applicationsWB, ELISA, ICC/IF more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic non-phosphopeptide derived from human PDCD4 around the phosphorylation site of serine 457
- Positive control
- 293 cells; HeLa cells.
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
- Concentration information loading...
- PurityImmunogen affinity purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab63359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||WB: 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).|
|ICC/IF||ICC/IF: 1/500 - 1/1000.|
- FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
- Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
- Sequence similaritiesBelongs to the PDCD4 family.
Contains 2 MI domains.
- DomainBinds EIF4A1 via both MI domains.
modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
- Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
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Anti-PDCD4 antibody images
All lanes : Anti-PDCD4 antibody (ab63359) at 1/500 dilution
Lane 1 : extracts from 293 cells
Lane 2 : extracts from 293 cells, with immunizing peptide
Predicted band size : 52 kDa
Observed band size : 52 kDa
Additional bands at : 56 kDa. We are unsure as to the identity of these extra bands.
Immunofluorescence analysis of PDCD4 expression in HeLa cells, using 1/500 ab63359. Left: untreated cells. Right: cells treated with immunising peptide.
References for Anti-PDCD4 antibody (ab63359)
ab63359 has not yet been referenced specifically in any publications.