Overview

  • Product nameAnti-PDCD4 antibodySee all PDCD4 primary antibodies ...
  • Description
    Mouse polyclonal to PDCD4
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human PDCD4 protein (AAH26104.1).

  • Positive control
    • Human pancreas lysate, PDCD4 transfected 293T cell lysate, HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab89940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • Sequence similaritiesBelongs to the PDCD4 family.
    Contains 2 MI domains.
  • DomainBinds EIF4A1 via both MI domains.
  • Post-translational
    modifications
    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

Anti-PDCD4 antibody images

  • Anti-PDCD4 antibody (ab89940) at 1 µg/ml + human pancreas lysate at 50 µg
    developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • All lanes : Anti-PDCD4 antibody (ab89940) at 1 µg/ml

    Lane 1 : PDCD4 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    developed using the ECL technique

    Predicted band size : 52 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ab89940, at 10 µg/ml, staining PDCD4 in HeLa cell by Immunofluorescence.

References for Anti-PDCD4 antibody (ab89940)

ab89940 has not yet been referenced specifically in any publications.

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