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Paraffin embedded human breast carcinoma tissue stained with ab74141 at a 1/50 dilution.
1: No phosphopeptide
2: Phosphopeptide present
All lanes : Anti-PDCD4 (phospho S457) antibody (ab74141) at 1/500 dilution
Lane 1 : extracts from 293 cells treated with serum (20%, 15mins)
Lane 2 : extracts from 293 cells treated with serum (20%, 15mins) with phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 52 kDa
Observed band size : 52 kDa
1
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