Anti-PDCD4 (phospho S457) antibody (ab74141)

Overview

• Product nameAnti-PDCD4 (phospho S457) antibodySee all PDCD4 primary antibodies ...
• Description
  Rabbit polyclonal to PDCD4 (phospho S457)
• Specificityab74141 detects endogenous levels of PDCD4 when phosphorylated at serine 457.
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthesized phosphopeptide derived from human PDCD4 around the phosphorylation site of serine 457 (F-V-S^P-E-G).

• Positive controlIHC-P: Human breast carcinoma tissue. WB: 293 cells treated with serum (20%, 15mins).

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThe antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Other

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Other

  • Cell Biology
  • Apoptosis
  • Nucleus
  • Other

Applications

Target

• FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
• Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
• Sequence similaritiesBelongs to the PDCD4 family.
  Contains 2 MI domains.
• DomainBinds EIF4A1 via both MI domains.
• Post-translational
  modificationsPolyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
• Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.

Target information above from: UniProt accession Q53EL6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 27250 Human
  • Entrez Gene: 18569 Mouse
  • Entrez Gene: 64031 Rat
  • Omim: 608610 Human
  • SwissProt: Q53EL6 Human
  • SwissProt: Q61823 Mouse
  • SwissProt: Q9JID1 Rat
  • Unigene: 711490 Human

  • Unigene: 1605 Mouse
  • Unigene: 375091 Mouse
  • Unigene: 206228 Rat

  see all

• Alternative names
  Death up-regulated gene protein antibodyDug antibodyH731 antibody

  Ma3 antibodyMGC33046 antibodyMGC33047 antibodyNeoplastic transformation inhibitor antibodyNeoplastic transformation inhibitor protein antibodyNuclear antigen H731 antibodyNuclear antigen H731 like antibodyNuclear antigen H731 like protein antibodyNuclear antigen H731-like antibodyPDCD 4 antibodyPdcd4 antibodyPDCD4_HUMAN antibodyProgrammed cell death 4 antibodyprogrammed cell death 4 (neoplastic transformation inhibitor) antibodyProgrammed cell death protein 4 antibodyProtein 197/15a antibodyProtein MA-3 antibodyRP11 348N5.4 antibodyTis antibodyTopoisomerase-inhibitor suppressed protein antibody

  see all

Anti-PDCD4 (phospho S457) antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 (phospho S457) antibody (ab74141)
  Paraffin embedded human breast carcinoma tissue stained with ab74141 at a 1/50 dilution.
  1: No phosphopeptide
  2: Phosphopeptide present
• 

  Western blot - PDCD4 (phospho S457) antibody (ab74141)
  All lanes : Anti-PDCD4 (phospho S457) antibody (ab74141) at 1/500 dilution
  
  Lane 1 : extracts from 293 cells treated with serum (20%, 15mins)
  Lane 2 : extracts from 293 cells treated with serum (20%, 15mins) with phosphopeptide at 5 µg
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 52 kDa
  Observed band size : 52 kDa