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Anti-PDCD4 (phospho S457) antibody (ab74141)

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Overview

Product name

Anti-PDCD4 (phospho S457) antibody
See all PDCD4 products (15) ...

Description

Rabbit polyclonal to PDCD4 (phospho S457)

Specificity

ab74141 detects endogenous levels of PDCD4 when phosphorylated at serine 457.

Tested applications

WB, ELISA, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthesized phosphopeptide derived from human PDCD4 around the phosphorylation site of serine 457 (F-V-SP-E-G).

Positive control

IHC-P: Human breast carcinoma tissue. WB: 293 cells treated with serum (20%, 15mins).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

The antibody was purified by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 (phospho S457) antibody (ab74141)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 (phospho S457) antibody (ab74141) image (enlarge)

  • Western blot - PDCD4 (phospho S457) antibody (ab74141)Western blot - PDCD4 (phospho S457) antibody (ab74141) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab74141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.

Tissue specificity

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.

Sequence similarities

Belongs to the PDCD4 family.
Contains 2 MI domains.

Domain

Binds EIF4A1 via both MI domains.

Post-translational
modifications

Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.

Cellular localization

Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.

Target information above from: UniProt accessionQ53EL6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Death up-regulated gene protein antibody
  • Dug antibody
  • H731 antibody
  • Ma3 antibody
  • MGC33046 antibody
  • MGC33047 antibody
  • Neoplastic transformation inhibitor antibody
  • Neoplastic transformation inhibitor protein antibody
  • Nuclear antigen H731 antibody
  • Nuclear antigen H731 like antibody
  • Nuclear antigen H731 like protein antibody
  • Nuclear antigen H731-like antibody
  • PDCD 4 antibody
  • Pdcd4 antibody
  • PDCD4_HUMAN antibody
  • Programmed cell death 4 antibody
  • programmed cell death 4 (neoplastic transformation inhibitor) antibody
  • Programmed cell death protein 4 antibody
  • Protein 197/15a antibody
  • Protein MA-3 antibody
  • RP11 348N5.4 antibody
  • Tis antibody
  • Topoisomerase-inhibitor suppressed protein antibody
see all

Database links

see all

Anti-PDCD4 (phospho S457) antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 (phospho S457) antibody (ab74141)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD4 (phospho S457) antibody (ab74141)

Paraffin embedded human breast carcinoma tissue stained with ab74141 at a 1/50 dilution.
1: No phosphopeptide
2: Phosphopeptide present

  Western blot - PDCD4 (phospho S457) antibody (ab74141)

Western blot - PDCD4 (phospho S457) antibody (ab74141)

All lanes : Anti-PDCD4 (phospho S457) antibody (ab74141) at 1/500 dilution

Lane 1 : extracts from 293 cells treated with serum (20%, 15mins)
Lane 2 : extracts from 293 cells treated with serum (20%, 15mins) with phosphopeptide at 5 µg

Lysates/proteins at 5 µg per lane.


Predicted band size : 52 kDa
Observed band size : 52 kDa

References for Anti-PDCD4 (phospho S457) antibody (ab74141)

ab74141 has not yet been referenced specifically in any publications.

Publishing research using ab74141? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"