Anti-PDCD4 (phospho S457) antibody (ab74141)

Overview

  • Product nameAnti-PDCD4 (phospho S457) antibody
    See all PDCD4 primary antibodies
  • Description
    Rabbit polyclonal to PDCD4 (phospho S457)
  • Specificityab74141 detects endogenous levels of PDCD4 when phosphorylated at serine 457.
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human PDCD4 around the phosphorylation site of serine 457 (F-V-SP-E-G).

  • Positive control
    • IHC-P: Human breast carcinoma tissue. WB: 293 cells treated with serum (20%, 15mins).

Properties

Applications

Our Abpromise guarantee covers the use of ab74141 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.
ELISA 1/10000.
IHC-P 1/50 - 1/100.

Target

  • FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • Sequence similaritiesBelongs to the PDCD4 family.
    Contains 2 MI domains.
  • DomainBinds EIF4A1 via both MI domains.
  • Post-translational
    modifications
    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • RP11 348N5.4 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

Anti-PDCD4 (phospho S457) antibody images

  • Paraffin embedded human breast carcinoma tissue stained with ab74141 at a 1/50 dilution.
    1: No phosphopeptide
    2: Phosphopeptide present
  • All lanes : Anti-PDCD4 (phospho S457) antibody (ab74141) at 1/500 dilution

    Lane 1 : extracts from 293 cells treated with serum (20%, 15mins)
    Lane 2 : extracts from 293 cells treated with serum (20%, 15mins) with phosphopeptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa

References for Anti-PDCD4 (phospho S457) antibody (ab74141)

This product has been referenced in:
  • Fay MM  et al. Enhanced Arginine Methylation of Programmed Cell Death 4 Protein during Nutrient Deprivation Promotes Tumor Cell Viability. J Biol Chem 289:17541-17552 (2014). Human . Read more (PubMed: 24764298) »

See 1 Publication for this product

Product Wall

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If you don't feel the vial of ab74141 you received is working as expected, I'd be happy to offer a replacement, credit or refund if you have submitted this review within about 6 months of receiving the antibody.
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Thank you for submitting your Abreview. I hope you have been notified it has been published on our website. I wanted to follow up with you regarding your review as your rating of 'Poor' indicated you were unhappy with the performance of ab74141. Please...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (embryonic pancreas)
Specification embryonic pancreas
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: glycine buffer pH 9
Permeabilization No
Blocking step cas-block (invitrogen) as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
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Verified customer

Submitted Jan 11 2012

Thank you for providing those extra details. The discount code generated is : xxxxx, expiration date: xxxxxxx I am very pleased to hear your customer would like to accept our offer and test ab74141 in mouse IHC. This code will give you 1 free primar...

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Thank you for your interest in the testing discount. We would be interested in your customer testing ab74141 in IHC-P with mouse sections. If you could please provide the following details I will generate a discount code for your customer to use: ...

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