Overview

  • Product nameAnti-PDCD4 (phospho S67) antibody
    See all PDCD4 primary antibodies
  • Description
    Rabbit polyclonal to PDCD4 (phospho S67)
  • Specificityab73343 detects endogenous levels of PDCD4 only when phosphorylated at serine 67.
  • Tested applicationsWB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PDCD4 around the phosphorylation site of serine 67 (KNSPSR)

  • Positive control
    • HepG2 cell extract. This antibody gave a positive result in IHC in the following FFPE tissue: Human pancreas.

Properties

Applications

Our Abpromise guarantee covers the use of ab73343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
ELISA 1/5000.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionInhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.
  • Tissue specificityUp-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.
  • Sequence similaritiesBelongs to the PDCD4 family.
    Contains 2 MI domains.
  • DomainBinds EIF4A1 via both MI domains.
  • Post-translational
    modifications
    Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.
  • Information by UniProt
  • Database links
  • Alternative names
    • Death up-regulated gene protein antibody
    • Dug antibody
    • H731 antibody
    • Ma3 antibody
    • MGC33046 antibody
    • MGC33047 antibody
    • Neoplastic transformation inhibitor antibody
    • Neoplastic transformation inhibitor protein antibody
    • Nuclear antigen H731 antibody
    • Nuclear antigen H731 like antibody
    • Nuclear antigen H731 like protein antibody
    • Nuclear antigen H731-like antibody
    • PDCD 4 antibody
    • Pdcd4 antibody
    • PDCD4_HUMAN antibody
    • Programmed cell death 4 antibody
    • programmed cell death 4 (neoplastic transformation inhibitor) antibody
    • Programmed cell death protein 4 antibody
    • Protein 197/15a antibody
    • Protein MA-3 antibody
    • RP11 348N5.4 antibody
    • Tis antibody
    • Topoisomerase-inhibitor suppressed protein antibody
    see all

Anti-PDCD4 (phospho S67) antibody images

  • All lanes : Anti-PDCD4 (phospho S67) antibody (ab73343) at 1/500 dilution

    Lane 1 : HepG2 cell extract
    Lane 2 : HepG2 cell extract with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
    Observed band size : 52 kDa
  • IHC image of PDCD4 (phospho S67) staining in Human normal pancreas formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73343, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-PDCD4 (phospho S67) antibody (ab73343)

This product has been referenced in:
  • Ge C  et al. Atheroprotective pulsatile flow induces ubiquitin-proteasome-mediated degradation of programmed cell death 4 in endothelial cells. PLoS One 9:e91564 (2014). Read more (PubMed: 24626527) »
  • Schmid T  et al. Inflammation-induced loss of Pdcd4 is mediated by phosphorylation-dependent degradation. Carcinogenesis 32:1427-33 (2011). Read more (PubMed: 21771721) »

See all 3 Publications for this product

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