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Anti-PDCD4 (phospho S67) antibody (ab73343)

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Overview

Product name

Anti-PDCD4 (phospho S67) antibody
See all PDCD4 products (15) ...

Description

Rabbit polyclonal to PDCD4 (phospho S67)

Specificity

ab73343 detects endogenous levels of PDCD4 only when phosphorylated at serine 67.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic phosphopeptide derived from human PDCD4 around the phosphorylation site of serine 67 (KNSPSR)

Positive control

HepG2 cell extract.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab73343 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Cell Biology >> Apoptosis >> Nucleus >> Other
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other

  • Western blot - PDCD4 (phospho S67) antibody (ab73343)Western blot - PDCD4 (phospho S67) antibody (ab73343) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab73343 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

Target

Function

Inhibits translation initiation and cap-dependent translation. May excert its function by hindering the interaction between EIF4A1 and EIF4G. Inhibits the helicase activity of EIF4A. Modulates the activation of JUN kinase. Down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. May play a role in apoptosis. Tumor suppressor. Inhibits tumor promoter-induced neoplastic transformation. Binds RNA.

Tissue specificity

Up-regulated in proliferative cells. Highly expressed in epithelial cells of the mammary gland. Reduced expression in lung cancer and colon carcinoma.

Sequence similarities

Belongs to the PDCD4 family.
Contains 2 MI domains.

Domain

Binds EIF4A1 via both MI domains.

Post-translational
modifications

Polyubiquitinated, leading to its proteasomal degradation. Rapidly degraded in response to mitogens. Phosphorylation of the phosphodegron promotes interaction with BTRC and proteasomal degradation.

Cellular localization

Nucleus. Cytoplasm. Shuttles between the nucleus and cytoplasm. Predominantly nuclear under normal growth conditions, and when phosphorylated at Ser-457. Exported from the nucleus in the absence of serum.

Target information above from: UniProt accessionQ53EL6 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Death up-regulated gene protein antibody
  • Dug antibody
  • H731 antibody
  • Ma3 antibody
  • MGC33046 antibody
  • MGC33047 antibody
  • Neoplastic transformation inhibitor antibody
  • Neoplastic transformation inhibitor protein antibody
  • Nuclear antigen H731 antibody
  • Nuclear antigen H731 like antibody
  • Nuclear antigen H731 like protein antibody
  • Nuclear antigen H731-like antibody
  • PDCD 4 antibody
  • Pdcd4 antibody
  • PDCD4_HUMAN antibody
  • Programmed cell death 4 antibody
  • programmed cell death 4 (neoplastic transformation inhibitor) antibody
  • Programmed cell death protein 4 antibody
  • Protein 197/15a antibody
  • Protein MA-3 antibody
  • RP11 348N5.4 antibody
  • Tis antibody
  • Topoisomerase-inhibitor suppressed protein antibody
see all

Database links

see all

Anti-PDCD4 (phospho S67) antibody images:

  Western blot - PDCD4 (phospho S67) antibody (ab73343)

Western blot - PDCD4 (phospho S67) antibody (ab73343)

All lanes : Anti-PDCD4 (phospho S67) antibody (ab73343) at 1/500 dilution

Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising phosphopeptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 52 kDa
Observed band size : 52 kDa

References for Anti-PDCD4 (phospho S67) antibody (ab73343)

This product has been referenced in:

  • Schmid Tet al. Inflammation-induced loss of Pdcd4 is mediated by phosphorylation-dependent degradation. Carcinogenesis 32:1427-33 (2011).Read more (PubMed: 21771721) »
  • Wang Xet al. US3 protein kinase of HSV-1 cycles between the cytoplasm and nucleus and interacts with programmed cell death protein 4 (PDCD4) to block apoptosis. Proc Natl Acad Sci U S A 108:14632-6 (2011).Read more (PubMed: 21844356) »

See all 2 publications for this product

Publishing research using ab73343? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"