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Western blot - PDCD5 antibody (ab75430)
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Immunocytochemistry/ Immunofluorescence - PDCD5 antibody (ab75430)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD5 antibody (ab75430)
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Product Name
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PDCD5 antibody
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See all PDCD5 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to PDCD5
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PDCD5.
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
IHC-P: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive signal in HEK293 whole cell lysate.
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Cellular localization
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Cytoplasmic and Nuclear.
Prior to apoptosis induction PDCD5 expression is both nuclear and cytoplasmic. Once apoptosis is induced it accumulates in the nucleus.
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Research areas
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Cancer >> Invasion/microenvironment >> Apoptosis >> Other
Cell Biology >> Apoptosis >> Intracellular >> Associated Proteins
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Relevance
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PDCD5 encodes a protein expressed in tumor cells during apoptosis independent of the apoptosis-inducing stimuli. Although its exact function is not defined, this protein is thought to play an early and universal role in apoptosis.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PDCD5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for PDCD5 antibody images, references, products related to ab75430 and other tools.
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PDCD5 antibody images:
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 Western blot - PDCD5 antibody (ab75430)
PDCD5 antibody (ab75430) at 1 µg/ml + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 14 kDa
Observed band size : 14 kDa
Additional bands at : 100 kDa,52 kDa,72 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
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Immunocytochemistry/ Immunofluorescence - PDCD5 antibody (ab75430)
ICC/IF image of ab75430 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75430, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDCD5 antibody (ab75430)
IHC image of ab75430 staining in Human Colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75430, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Search PubMed (MEDLINE) for references to PDCD5
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PDCD5 antibody - more information
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Customer reviews (feedback) regarding PDCD5 antibody |
Customer FAQs regarding PDCD5 antibody |
Protocols for PDCD5 antibody |
Price and availability of products related to PDCD5 antibody |
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PDCD5 antibody - related products:
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PDCD5 antibody - other tools:
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Contact Abcam with a Technical Enquiry about PDCD5 antibody |
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