PDE3B antibody (ab42091)

Product Name 

PDE3B antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to PDE3B

Immunogen 

Synthetic peptide (Human) (selective for only PDE3B variant).

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

Does not cross react with PDE4A variants nor with any other PDEs.

Tested applications 
(see key)

IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IP: 1/200.
WB: 1/500. Detects a band of approximately 145 kDa (predicted molecular weight: 145 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; peripheral membrane protein

Research areas 

Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> cGMP
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> cAMP

Relevance 

Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents. The members of the PDE3 families are known as cGMP inhibited PDEs. The PDE3 enzymes bind both cGMP and cAMP with different affinities (cAMP 4-10-fold than cGMP). The phosphodiesterase type-3 (PDE3) family is comprised of 2 genes (PDE3A and PDE3B). PDE3A is a 145 kDa protein and is expressed in various tissues (myocardium, vascular and non vascular smooth muscles, megakaryocytes and a subset of neurons (Snyder 1999; Maecci et al., 1992). PDE3B is 1112 amino acids long and has two splice variants which are expressed in adipocyte tissue, hepatocyte, kidney, spermatocytes and embryonic neuroepithelium (Reinhatdt et. al., 1995). Both PDE3A and PDE3B are therapeutic targets for the treatment of obesity as inhibition of PDE3 increase the lipolytic activity and alter the insulin secretion and its response. PDE3 inhibitors also promote thermogenic and cardiotonic effects in animals.

Database links 

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Antibody stabilization buffer, with preservatives, consisting of Hepes, NaCl, KCl, glycerol, BSA and chaotropic agents.

Form 

Liquid

Concentration 

Batch dependent within range: 0.77 - 1.10 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Store at -20°C, Stable for 6 months at -20°C

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