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Western blot - PDE3B antibody (ab55558)
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Product Name
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PDE3B antibody
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See all PDE3B antibodies (2)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to PDE3B
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Immunogen
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Recombinant fragment: LTPFPGFYPC SEIEDPAEKG DRKLNKGLNR NSLPTPQLRR SSGTSGLLPV EQSSRWDRNN GKRPHQEFGI SSQGCYLNGP FNSNLLTIPK QRSSSVSLTH , corresponding to amino acids 401-501 of Human PDE3B
BLAST 'LTPFPGFYPC SEIEDPAEKG DRKLNKGLNR NSLPTPQLRR SSGTSGLLPV EQSSRWDRNN GKRPHQEFGI SSQGCYLNGP FNSNLLTIPK QRSSSVSLTH ' with  or 
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Reacts with
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Hu
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1-5 µg/ml.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Membrane; peripheral membrane protein
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Research areas
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Signal Transduction >> Metabolism >> Lipid metabolism
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> cGMP
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> cAMP
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Relevance
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Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-PDE11) based on their kinetic properties and inhibition to various pharmacological agents. The members of the PDE3 families are known as cGMP inhibited PDEs. The PDE3 enzymes bind both cGMP and cAMP with different affinities (cAMP 4-10-fold than cGMP). The phosphodiesterase type-3 (PDE3) family is comprised of 2 genes (PDE3A and PDE3B). PDE3A is a 145 kDa protein and is expressed in various tissues (myocardium, vascular and non vascular smooth muscles, megakaryocytes and a subset of neurons (Snyder 1999; Maecci et al., 1992). PDE3B is 1112 amino acids long and has two splice variants which are expressed in adipocyte tissue, hepatocyte, kidney, spermatocytes and embryonic neuroepithelium (Reinhatdt et. al., 1995). Both PDE3A and PDE3B are therapeutic targets for the treatment of obesity as inhibition of PDE3 increase the lipolytic activity and alter the insulin secretion and its response. PDE3 inhibitors also promote thermogenic and cardiotonic effects in animals.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2a
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Light chain type
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kappa
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
PBS, pH 7.2
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PDE3B antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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PDE3B antibody images:
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 Western blot - PDE3B antibody (ab55558)
Western blot against tagged recombinant protein immunogen using ab55558 PDE3B antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa
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Search PubMed (MEDLINE) for references to PDE3B
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PDE3B antibody - more information
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Customer reviews (feedback) regarding PDE3B antibody |
Customer FAQs regarding PDE3B antibody |
Protocols for PDE3B antibody |
Price and availability of products related to PDE3B antibody |
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PDE3B antibody - other tools:
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Contact Abcam with a Technical Enquiry about PDE3B antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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