PDE6 delta protein (T7 Tag) (ab40492)

Product Name 

PDE6 delta protein (T7 Tag)

Product type 

Proteins and Peptides

Peptide 

Recombinant full length protein (Human).
Fusion: T7 tag at the N terminus.

Tested applications 
(see key)

MS, SDS-PAGE

Application notes 
(see key)

Recommended dilutions
MS: Use at an assay dependent dilution.
SDS-PAGE: Use at an assay dependent dilution.
This peptide can be used with studies using ab37662.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> cGMP
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> cAMP

Relevance 

The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways.

PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha’ (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha’/alpha’ homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: 0.002% Sodium Azide
Constituents: 10mM Tris, 10mM DTT, 0.1% Triton X-100, pH 8

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.700 mg/ml

Storage instructions 

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Expression system 

E. coli

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