Anti-PDF antibody (ab68821)

Overview

• Product nameAnti-PDF antibodySee all PDF primary antibodies ...
• Description
  Mouse polyclonal to PDF
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Full length human PDF protein (AAH19912)

• Positive controlHuman spleen tissue lysate; PDF transfected 293T cell line

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: Whole serum
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Amino acid metabolism

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial translation

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Nucleotide metabolism

  • Signal Transduction
  • Metabolism
  • Amino Acids

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • Translation
  • Mito. Translation

  • Cell Biology
  • Cell Cycle
  • Cell Division
  • Other cell division antibodies

Applications

Target

• FunctionRemoves the formyl group from the N-terminal Met of newly synthesized proteins.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the polypeptide deformylase family.
• Cellular localizationMitochondrion.

Target information above from: UniProt accession Q9HBH1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 64146 Human
  • SwissProt: Q9HBH1 Human
  • Unigene: 130849 Human

• Alternative names
  CG6496 antibodyDEFM_HUMAN antibodymitochondrial antibody

  Pdf antibodyPDF1A antibodyPeptide deformylase antibodyPeptide deformylase like protein antibodyPeptide deformylase, mitochondrial antibodyPolypeptide deformylase antibody

  see all

Anti-PDF antibody images

• 

  Western blot - PDF antibody (ab68821)
  Anti-PDF antibody (ab68821) at 1/500 dilution + human spleen tissue lysate at 25 µg
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
  
  Predicted band size : 27 kDa
  Observed band size : 32 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - PDF antibody (ab68821)
  All lanes : Anti-PDF antibody (ab68821) at 1/500 dilution
  
  Lane 1 : PDF transfected 293T cell line
  Lane 2 : non transfected 293T cell line
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
  
  Predicted band size : 27 kDa
  Observed band size : 25 kDa (why is the actual band size different from the predicted?)