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Product Name
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PDGF AB + BB antibody
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Product type
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Primary antibodies
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Description
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Goat polyclonal to PDGF AB + BB
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Immunogen
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Purified human PDGF-AB
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Reacts with
(species key)
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Hu, Ms, Rat, Cow, Pig
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Specificity
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Recognizes non-reduced human PDGF AA, AB and BB dimers in western immunoblots. Porcine and bovine PDGF may be detected with decreased sensitivity. Rodent PDGF will not be detected. This antibody will neutralize human, porcine, bovine and rodent PDGF.
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Tested applications
(see key)
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Neut, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Neut: Use at a concentration of 10 - 20 µg/ml. In a 5 day assay, 10-20ug/ml inhibited 3T3 cell growth by 60-75% as stimulated by 10ng/ml of PDGF-AB.
WB: Use at a concentration of 5 - 10 µg/ml. Predicted molecular weight: 124 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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3T3 cells
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Cellular localization
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Membrane; single pass type I membrane protein.
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Research areas
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Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Growth factors >> PDGF
Signal Transduction >> Growth Factors/Hormones >> PDGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Cardiovascular >> Blood >> Serum Proteins
Cardiovascular >> Angiogenesis >> Growth Factors >> Other
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Relevance
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Platelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.
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PDGF AB + BB antibody (ab34074) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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IgG fraction
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Storage buffer
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Preservative: None
Constituents: 40% Glycerol, 0.1M Tris glycine. pH 7.0 Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Purification notes
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Purified by DEAE chromatography.
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Form
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Liquid
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Concentration
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2.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this PDGF AB + BB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to PDGF AB + BB
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PDGF AB + BB antibody - more information
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Customer reviews (feedback) regarding PDGF AB + BB antibody |
Customer FAQs regarding PDGF AB + BB antibody |
Protocols for PDGF AB + BB antibody |
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