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Products:Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Overview
Product name
Anti-PDGF AB + BB antibody
Description
Goat polyclonal to PDGF AB + BB
Specificity
Recognizes non-reduced human PDGF AA, AB and BB dimers in western immunoblots. Porcine and bovine PDGF may be detected with decreased sensitivity. Rodent PDGF will not be detected. This antibody will neutralize human, porcine, bovine and rodent PDGF.
Tested applications
WB, Neutralisingmore details
Cross reactivity
Reacts with
Mouse, Rat, Cow, Human, Pig
Immunogen
Purified human PDGF-AB
Positive control
3T3 cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 40% Glycerol, 0.1M Tris glycine. pH 7.0
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
Purified by DEAE chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cancer >> Oncoproteins/suppressors >> Oncoproteins >> Growth factor receptors
Cancer >> Growth factors >> PDGF
Signal Transduction >> Growth Factors/Hormones >> PDGF
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Receptor Tyrosine Kinases
Cardiovascular >> Blood >> Serum Proteins
Cardiovascular >> Angiogenesis >> Growth Factors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab34074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Neutralising
Application notes
Neut: Use at a concentration of 10 - 20 µg/ml. In a 5 day assay, 10-20ug/ml inhibited 3T3 cell growth by 60-75% as stimulated by 10ng/ml of PDGF-AB.
WB: Use at a concentration of 5 - 10 µg/ml. Predicted molecular weight: 124 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Platelet-Derived Growth Factor (PDGF) is the major serum mitogen for cells of mesenchymal origin in humans. The biologically active protein is a dimer composed of two related polypeptides designated A and B. The PDGF protein has been implicated both directly as well as indirectly in several pathological states including neoplasia, arthritis, arteriosclerosis and bone marrow sclerosis.
Cellular localization
Secreted
Alternative names
- Becaplermin antibody
- c sis antibody
- FLJ1285 antibody
see all
Database links
- Entrez Gene: 5154 Human
- Entrez Gene: 5155 Human
- Entrez Gene: 18590 Mouse
- Entrez Gene: 18591 Mouse
- Entrez Gene: 24628 Rat
- Entrez Gene: 25266 Rat
- Omim: 173430 Human
- Omim: 190040 Human
see all
References for Anti-PDGF AB + BB antibody (ab34074)
This product has been referenced in:
- Song CHet al. Identification of chemoattractive factors involved in the migration of bone marrow-derived mesenchymal stem cells to brain lesions caused by prions. J Virol 85:11069-78 (2011).Read more (PubMed: 21813601) »
See 1 publication for this product
Publishing research using ab34074? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"