Human PDGF AB ELISA Kit (ab100623)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 10.24 pg/ml - 2500 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human PDGF AB ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
10.24 pg/ml - 2500 pg/ml -
Recovery
74 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 74.29 68% - 78% Serum 73.96 67% - 78% Plasma 74.85 67% - 79% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s PDGF AB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human PDGF AB in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human PDGF AB coated on a 96- well plate. Standards and samples are pipetted into the wells and PDGF AB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human PDGF AB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF AB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 500X HRP-Streptavidin Concentrate 1 x 200µl Assay Diluent A 1 x 30ml Biotinylated anti-Human PDGF AB 2 vials PDGF AB Microplate (12 x 8 wells) 1 unit Recombinant Human PDGF AB Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains joined by disulphide bonds, termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Human PDGF-AB is a 25.5 kDa heterodimer protein consisting of a 13.3 kDa A chain and a 12.2 kDa B chain. -
Alternative names
- PDGF A chain
- PDGF B chain
- PDGF1
see all -
Database links
- Entrez Gene: 5154 Human
- Entrez Gene: 5155 Human
- SwissProt: P04085 Human
- SwissProt: P01127 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab100623 has not yet been referenced specifically in any publications.