PDGF BB Human ELISA Kit (ab100624)
- Product namePDGF BB Human ELISA KitSee all PDGF BB kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 1 pg/ml
- Range0.549 pg/ml - 400 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 95.43 84% - 104% Serum 93.57 82% - 103% Plasma 91.99 83% - 104%
- Species reactivityReacts with: Human
- Product overview
ab100624 PDGF BB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PDGF BB in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for human PDGF BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PDGF BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody PDGF-BB: biotinylated anti-human PDGF-BB 2 vials HRP-Streptavidin Concentrate 800x concentrated HRP-conjugated streptavidin 1 x 200µl PDGF BB Microplate (12 strips x 8 wells) coated with anti-human PDGF-BB 1 unit PDGF BB Standard Human(recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
- Becaplerminc sisFLJ12858
- Oncogene SIS PDG B chain PDGBPDGF 2PDGF BPDGF B chainPDGF subunit BPDGF-2PDGF2PdgfbPDGFB/COL1A1 fusion gene PDGFB_HUMANPlatelet derived growth factor 2Platelet derived growth factor B chainPlatelet derived growth factor beta polypeptidePlatelet derived growth factor beta polypeptide (oncogene SIS) Platelet derived growth factor subunit BPlatelet-derived growth factor B chainPlatelet-derived growth factor beta polypeptidePlatelet-derived growth factor subunit BProto oncogene c SisProto-oncogene c-SisSimian sarcoma viral (v sis) oncogene homologSISSSVV sis platelet derived growth factor beta polypeptide
Our Abpromise guarantee covers the use of ab100624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for PDGF BB Human ELISA Kit (ab100624)
ab100624 has not yet been referenced specifically in any publications.