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Overview

  • Product namePDGF BB Human ELISA KitSee all PDGF BB kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 1 pg/ml
  • Range
    0.549 pg/ml - 400 pg/ml
  • Recovery

    93 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 95.43 84% - 104%
    Serum 93.57 82% - 103%
    Plasma 91.99 83% - 104%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100624 PDGF BB Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PDGF BB in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for human PDGF BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human PDGF BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • FunctionGrowth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.
  • Tissue specificityExpressed at high levels in the heart, brain (sustantia nigra), placenta and fetal kidney. Expressed at moderate levels in the brain (hippocampus), skeletal muscle, kidney and lung.
  • Involvement in diseaseNote=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.
  • Sequence similaritiesBelongs to the PDGF/VEGF growth factor family.
  • Cellular localizationSecreted. Released by platelets upon wounding.

    • Target information above from: UniProt accession P01127 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Becaplerminc sisFLJ12858
        Oncogene SIS PDG B chain PDGBPDGF 2PDGF BPDGF B chainPDGF subunit BPDGF-2PDGF2PdgfbPDGFB/COL1A1 fusion gene PDGFB_HUMANPlatelet derived growth factor 2Platelet derived growth factor B chainPlatelet derived growth factor beta polypeptidePlatelet derived growth factor beta polypeptide (oncogene SIS) Platelet derived growth factor subunit BPlatelet-derived growth factor B chainPlatelet-derived growth factor beta polypeptidePlatelet-derived growth factor subunit BProto oncogene c SisProto-oncogene c-SisSimian sarcoma viral (v sis) oncogene homologSISSSVV sis platelet derived growth factor beta polypeptide
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100624 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      PDGF BB Human ELISA Kit images

      • ELISA - PDGF BB Human ELISA Kit (ab100624)
        ELISA - PDGF BB Human ELISA Kit (ab100624)

      Protocols

      References for PDGF BB Human ELISA Kit (ab100624)

      ab100624 has not yet been referenced specifically in any publications.

      Publishing research using ab100624 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100624 Submit a Question for ab100624

      Displaying 1 - 8 of 8 results for Abreviews and Q&A

      10
      Votes: Highest First

      Thanks a lot again for your reply regarding some modifications for PDGF-BB Kit (abcam 100624) on 11/19/12.

      The second experiment using this kit was performed with your suggestions; overnigh incubation at 4 oC instead of 2.5 hours at room temp.;...

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      Thank you very much for your reply. My colleague is out of the office but I'm happy to help you with this issue.

      Your credit note ID is *** I am sorry that thiskit did not perform as expected. If payment has already been made on the original or...

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      The next time we will try (2) new things ; concentrating or reducing volume to 50% by using ultracentrifuge filter units and overnight incubation (instead of 2.5 hours incubation).

      Thw Molecular Weight for PDGF-BB is 24.3 KDa. Do you have any s...

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      Thank you for your reply.

      We do have 10 kDa spin colums for ultrafiltration available as ab93349 and concentration kits available, as well. I'm not exactly sure how you would like to concentrate your samples, but here is the link to our current ...

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      No signal/weak signal from samples of interest (cell culture supernatent) but got signal from the standards.
      1 - Will FCS interfer with the assay signal?
      2 - Can you use froxen supernatent or does it have to be fresh?
      3 - Signal was weak af...

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      Thank you for contacting Abcam.

      Please find the answers to your questions below

      1 - Will FCS interfer with the assay signal?
      No. However, since serum-containing media does contain cytokines, I would recommend that the you also run...

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      Yes please go ahead.

      Thank you once again for your enquiries.
      I can confirm this component has now been added as a separate item to the catalog.

      Please use this product code when placing your order:
      ab135400 HRP-Streptavidin Concentrate 800x concentrated ...

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      Can we purchase the HRP streptaviding concentrate from kit ab100624 separately? We have lost the vial.

      Thank you for your telephone call yesterday afternoon.

      I have contacted my colleagues who have informed me that we have some website and administration changes taking place this week, so regrettably I am not able to do this for you today. Howev...

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      Please send certificates of compliance for product #ab100624, lot# GR66784 and for product #ab100626, lot# GR72270



      Thank you!

      Thank you for contacting Abcam.

      Please find attached the Certificate of Compliances for ab100624 and ab100626.

      If there is anything else I can help you with, please do not hesitate to contact me.

      Please send certificates of compliance for cat#ab100626 and cat#ab100624.  Thank you.  

      Thank you for contacting Abcam. Attached, please find the Certificates of Compliance you requested. I hope this information is helpful.  Please do not hesitate to contact us if you have any additional questions.

      Dear technical team,   Our customer would like to purchase PDGF ELISA Kit from abcam.   http://www.abcam.com/index.html?pageconfig=searchresults&pt=4&search=pdgf   I have searched 5 products and the customer's sample is cell culture supe...

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      Thank you for your enquiry. From the search results you have provided, I can confirm that the following PDGF ELISA kits are tested on cell culture samples and will be suitable for this sample type. I can suggest to select one that has been tested ...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"