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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-PDGF BB antibody
See all PDGF BB products (10) ...
Description
Goat polyclonal to PDGF BB
Specificity
ab10845 will neutralize the biological activity of recombinant human PDGF BB. It will also neutralize the activity of recombinant human-AB, natural human PDGF, rat PDGF BB, and porcine PDGF BB. It will neutralize the activity of recombinant human PDGF AA at 50-100 fold higher IgG concentration. By immunoblotting, the antibody shows < 10% crossreactivity with recombinant human PDGF AA.
Tested applications
ELISA, WB, Neutralisingmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length protein (Human) expressed in E. coli.
Positive control
Tested in PDGF-responsive NR6R-3T3 fibroblasts.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody is purified using human PDGF BB affinity chromatography.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cancer >> Growth factors >> PDGF
Signal Transduction >> Growth Factors/Hormones >> PDGF
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10845 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
WB
Neutralising
Application notes
ELISA: Use at a concentration of 0.5-1 µg/ml.
Neut: ND50 is approximately 0.025 – 0.2 µg/ml.
WB: Use at a concentration of 0.1-0.2 µg/ml. Predicted molecular weight: 28 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Target information above from: UniProt accessionP01127
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Becaplermin antibody
- C sis antibody
- FLJ12858 antibody
see all
Database links
- Entrez Gene: 5155 Human
- Entrez Gene: 18591 Mouse
- Omim: 190040 Human
- SwissProt: P01127 Human
- SwissProt: P31240 Mouse
- Unigene: 1976 Human
- Unigene: 144089 Mouse
References for Anti-PDGF BB antibody (ab10845)
This product has been referenced in:
- Yamamoto Set al. Platelet-derived Growth Factor Receptor Regulates Salivary Gland Morphogenesis via Fibroblast Growth Factor Expression. J Biol Chem 283:23139-23149 (2008). WB; Mouse.Read more (PubMed: 18559345) »
See 1 publication for this product
Publishing research using ab10845? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"