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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-PDGF BB antibody
See all PDGF BB products (10) ...
Description
Rabbit polyclonal to PDGF BB
Specificity
This antibody has been tested against PDGF BB protein (ab9706) and has yet to be tested against endogenous protein.
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PDGF BB.
(Peptide available as ab24035.)
Positive control
PDGF BB protein (ab9706)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cancer >> Growth factors >> PDGF
Signal Transduction >> Growth Factors/Hormones >> PDGF
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 2 Images
WB
Application notes
IHC-P: 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14-18 kDa). Can be blocked with PDGF BB peptide (ab24035). The predicted molecular weight of PDGF BB is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF BB runs between 14-18 kDa on a gel.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Target information above from: UniProt accessionP01127
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Becaplermin antibody
- Becaplermin antibody
- C sis antibody
see all
Database links
- Entrez Gene: 5155 Human
- Entrez Gene: 18591 Mouse
- Entrez Gene: 24628 Rat
- Omim: 190040 Human
- SwissProt: P01127 Human
- SwissProt: P31240 Mouse
- SwissProt: Q05028 Rat
- Unigene: 1976 Human
see all
Anti-PDGF BB antibody images:
Western blot - PDGF BB antibody (ab23914)
All lanes : Anti-PDGF BB antibody (ab23914) at 1 µg/ml
Lane 1 : PDGF BB protein (Active) (ab9706)
Lane 2 : PDGF BB protein (Active) (ab9706) with PDGF BB peptide (ab24035) at 1 µg
Lysates/proteins at 0.01 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 14-18 kDa
Observed band size : 14 kDa (why is the actual band size different from the predicted?)
ab23914 detects a band of ~ 14 kDa corresponding to the reduced form of recombinant PDGF BB (ab9706). The predicted molecular weight for PDGF BB is 27 kDa according to the Swissprot entry, however the reduced form is known to run at between 14-18 kDa. The 14 kDa band is specifically blocked by the addition of the immunizing peptide, ab 24035.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab23914)
Image courtesy of Human Protein Atlas
ab23914 staining PDGF BB in Human kidney (male). The paraffin embedded tissue was incubated with ab23914 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23914 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.
Further results for this antibody can be found at www.proteinatlas.org
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab23914)
IHC image of PDGF BB staining in huan heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23914, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot
Anti-PDGF BB antibody (ab23914) at 1 µg/ml + PDGF BB protein (His tag) (ab73231) at 0.01 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
References for Anti-PDGF BB antibody (ab23914)
ab23914 has not yet been referenced specifically in any publications.
Publishing research using ab23914? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"