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Anti-PDGF BB antibody (ab23914)

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Overview

Product name

Anti-PDGF BB antibody
See all PDGF BB products (10) ...

Description

Rabbit polyclonal to PDGF BB

Specificity

This antibody has been tested against PDGF BB protein (ab9706) and has yet to be tested against endogenous protein.

Tested applications

WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human PDGF BB.

(Peptide available as ab24035.)

Positive control

PDGF BB protein (ab9706)

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - PDGF BB antibody (ab23914)Western blot - PDGF BB antibody (ab23914) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab23914)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab23914) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab23914)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab23914) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab23914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 14-18 kDa). Can be blocked with PDGF BB peptide (ab24035). The predicted molecular weight of PDGF BB is ~ 27 kDa according to the Swissprot entry P01127, however the reduced form of PDGF BB runs between 14-18 kDa on a gel.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Target information above from: UniProt accessionP01127 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Becaplermin antibody
  • Becaplermin antibody
  • C sis antibody
  • C sis antibody
  • FLJ12858 antibody
  • Oncogene SIS antibody
  • Oncogene SIS antibody
  • PDG B chain antibody
  • PDG B chain antibody
  • PDGB antibody
  • PDGB antibody
  • PDGF 2 antibody
  • PDGF B antibody
  • PDGF B chain antibody
  • PDGF subunit B antibody
  • PDGF-2 antibody
  • PDGF2 antibody
  • PDGF2 antibody
  • PDGFB antibody
  • PDGFB/COL1A1 fusion gene antibody
  • PDGFB/COL1A1 fusion gene antibody
  • PDGFB_HUMAN antibody
  • Platelet derived growth factor 2 antibody
  • Platelet derived growth factor B chain antibody
  • Platelet derived growth factor B chain antibody
  • Platelet derived growth factor beta antibody
  • Platelet derived growth factor beta polypeptide antibody
  • Platelet derived growth factor beta polypeptide antibody
  • Platelet-derived growth factor B chain antibody
  • Platelet-derived growth factor beta polypeptide antibody
  • Platelet-derived growth factor subunit B antibody
  • Proto-oncogene c-Sis antibody
  • Simian sarcoma viral (v sis) oncogene homolog antibody
  • Simian sarcoma viral (v sis) oncogene homolog antibody
  • SIS antibody
  • SSV antibody
  • SSV antibody
  • V sis platelet derived growth factor beta polypeptide antibody
  • V sis platelet derived growth factor beta polypeptide antibody
see all

Anti-PDGF BB antibody images:

  Western blot - PDGF BB antibody (ab23914)

Western blot - PDGF BB antibody (ab23914)

All lanes : Anti-PDGF BB antibody (ab23914) at 1 µg/ml

Lane 1 : PDGF BB protein (Active) (ab9706)
Lane 2 : PDGF BB protein (Active) (ab9706) with PDGF BB peptide (ab24035) at 1 µg

Lysates/proteins at 0.01 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 14-18 kDa
Observed band size : 14 kDa (why is the actual band size different from the predicted?)


ab23914 detects a band of ~ 14 kDa corresponding to the reduced form of recombinant PDGF BB (ab9706). The predicted molecular weight for PDGF BB is 27 kDa according to the Swissprot entry, however the reduced form is known to run at between 14-18 kDa. The 14 kDa band is specifically blocked by the addition of the immunizing peptide, ab 24035.

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab23914)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab23914)

Image courtesy of Human Protein Atlas

ab23914 staining PDGF BB in Human kidney (male). The paraffin embedded  tissue was incubated with ab23914 (1/500 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab23914 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines.

Further results for this antibody can be found at www.proteinatlas.org

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab23914)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab23914)

IHC image of PDGF BB staining in huan heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab23914, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  Western blot

Western blot

Anti-PDGF BB antibody (ab23914) at 1 µg/ml + PDGF BB protein (His tag) (ab73231) at 0.01 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Exposure time : 1 minute

References for Anti-PDGF BB antibody (ab23914)

ab23914 has not yet been referenced specifically in any publications.

Publishing research using ab23914? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"