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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-PDGF BB antibody
See all PDGF BB products (10) ...
Description
Rabbit polyclonal to PDGF BB
Tested applications
WB, ELISA, Neutralising, IHC-Pmore details
Cross reactivity
Reacts with
Human
Immunogen
Highly pure (>98%) recombinant hPDGF-BB (human Platelet Derived Growth Factor)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Research areas
Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cancer >> Growth factors >> PDGF
Signal Transduction >> Growth Factors/Hormones >> PDGF
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9704 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent concentration. (To detect hPDGF-BB by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPDGF-BB is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.)
ELISA: Use at an assay dependent concentration. (To detect hPDGF-BB by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPDGF-BB.)
Neut: Use at an assay dependent concentration. (To yield one-half maximal inhibition [ND50] of the biological activity of hPDGF-BB (2.5 ng/ml), a concentration of 0.03 - 0.04 µg/ml of this antibody is required.)
IHC-P: Use a concentration of 1 µg/ml
Target
Target information above from: UniProt accessionP01127
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Becaplermin antibody
- C sis antibody
- FLJ12858 antibody
see all
Database links
- Entrez Gene: 5155 Human
- Omim: 190040 Human
- SwissProt: P01127 Human
- Unigene: 1976 Human
Anti-PDGF BB antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PDGF BB antibody (ab9704)
ab9704 staining PDGF BB in normal human skin tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). Tissue underwent proteinase K mediated antigen retrieval. The primary antibody was used at 1 ug/ml and incubated with sample at 4°C overnight. A HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen.
Western blot - PDGF BB antibody (ab9704)
Anti-PDGF BB antibody (ab9704) at 0.2 µg/ml + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab52255) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Observed band size : 18,29 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa (possible dimer).
The 29kDa form is the propeptide.
References for Anti-PDGF BB antibody (ab9704)
This product has been referenced in:
- Yang Jet al. Platelet-derived growth factor mediates survival of leukemic large granular lymphocytes via an autocrine regulatory pathway. Blood 115:51-60 (2010). Neutralising; Human.Read more (PubMed: 19880494) »
- Kim GIet al. Electrochemical detection of vascular endothelial growth factors (VEGFs) using VEGF antibody fragments modified Au NPs/ITO electrode. Biosens Bioelectron 25:1717-22 (2010).Read more (PubMed: 20047825) »
See all 2 publications for this product
Publishing research using ab9704? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"