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PDGF BB protein (Active) (ab51925)

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Overview

Product name

PDGF BB protein (Active)
See all PDGF BB products (9) ...

Protein description

Recombinant Mouse PDGF BB; a homodimeric, non-glycosylated, polypeptide of 109 amino acids, having a molecular mass of 24.4 kDa.

Expression host

E. coli

Properties

Purity

>95% by SDS-PAGE

Purification notes

>95% by SDS-PAGE and HPLC analyses. Endotoxin level is <0.1 ng per µg.

Form

Lyophilised:
Centrifuge the vial prior to opening. Reconstitute in sterile 100mM acetic acid and 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

Storage instructions

Store at -20°C.

Storage buffer

Preservative: None
Constituents: 0.1% BSA, 100mM Acetic acid

Concentration

Concentration information loading...

Additional notes

The lyophilized protein is best-stored desiccated below 0oC. Reconstituted human PDGF-BB should be stored in working aliquots at -20oC The reconstituted solution can be diluted into aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Research areas

Stem Cells >> Stem Cell Cytokines >> Recombinant Mouse
Cancer >> Cancer Metabolism >> Response to hypoxia
Developmental Biology >> Organogenesis >> Angiogenesis and vasculogenesis
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cancer >> Growth factors >> PDGF
Signal Transduction >> Growth Factors/Hormones >> PDGF
Cardiovascular >> Blood >> Serum Proteins

Applications

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Our Abpromise guarantee covers the use of ab51925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Functional Studies

     

  • SDS-PAGE

     

Application notes

Biological Activity: The ED50 as determined by the dose-dependent proliferation of murine BALB/c 3T3 cells is < 2 ng/ml.
SDS-PAGE: Use at an assay dependent dilution.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Protein info

Target information above from: UniProt accessionP01127 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

References for PDGF BB protein (Active) (ab51925)

ab51925 has not yet been referenced specifically in any publications.

Publishing research using ab51925? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"